Expression of pigment epithelium-derived factor in the colorectal cancer
Objective To investigate the expression of pigment epithelium-derived factor(PEDF)in the colorectal cancer and its correlation with clinicopathological parameters and prognosis.Methods The expression of PEDF mRNA and protein in colorectal cancer,lymph node metastatic cancer and normal intestinal mucosal tissues were assessed by real-time fluorescence quantitative PCR and immunohistochemical staining.The correlations between PEDF expression and clinico-pathological parameters or prognosis were analyzed.Results The relative expression level of PEDF mRNA was 0.115±0.023 in the colorectal cancer tissues,0.201±0.063 in the lymph node metastatic cancer tissues,and 0.821±0.076 in normal intestinal mucosa tissues,respectively(F=9.324,P=0.025).Furthermore,the relative expression levels of PEDF mRNA in the colorectal cancer tissues and the lymph node metastatic cancer tissues was lower than that in the normal intestinal mucosa tissues,and the relative expression level of PEDF mRNA in the colorectal cancer tissues was lower than that in the lymph node metastatic cancer tissues(P<0.001).Among colorectal cancer patients,the proportion of patients with low PEDF protein expression was 68.33%(82/120),and the proportion of patients with high PEDF protein expres-sion was 31.67%(38/120).The expression of PEDF protein in the colorectal cancer tissues was related to TNM stage and lymph node metastasis(χ2=57.449,P<0.001;χ2=50.612,P<0.001).The median survival time of patients with low PEDF protein expression was 30.82 months,which was lower than that of patients with high PEDF protein expression(46.89 months,Log-rank χ2=18.140,P<0.001).Conclusion The expression of PEDF is closely related to TNM stage and lymph node metastasis in patients with colorectal cancer,low expression of PEDF in colorectal cancer tissues is a risk factor for poor prognosis in colorectal cancer patients.
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