基于结肠癌组织芯片的腺苷酸活化蛋白激酶α表达及其意义初析
Preliminary analysis of expression and significance of adenosine monophosphate-acti-vated protein kinase α in the colon cancer based on tissue microarray
董俭达 1陈山 2徐秋燕 3王宝宏 4张艳冰 4张飞雄5
作者信息
- 1. 宁夏医科大学基础医学院病理学系,宁夏银川 750004;宁夏医科大学功能形态学实验室,宁夏银川 750004
- 2. 宁夏医科大学总医院心脑血管病医院血管外科,宁夏 银川 750002
- 3. 贺兰县人民医院,宁夏 贺兰 750200
- 4. 宁夏医科大学基础医学院病理学系,宁夏银川 750004
- 5. 宁夏医科大学总医院心脑血管病医院消化内科,宁夏 银川 750002
- 折叠
摘要
目的 利用结肠癌组织芯片,研究腺苷酸活化蛋白激酶α(AMPKα)在正常结肠黏膜、腺瘤、腺癌及转移性结肠癌组织中的表达及临床病理学意义.方法 采用 SABC免疫组织化学法检测结肠癌组织芯片组织中AMPKα蛋白的表达情况,并利用Image J软件对阳性强度及阳性率进行半定量分析.结果 与对应隐窝腺体比较,AMPKα在正常结肠黏膜中表面上皮的表达较高(t=3.053,P=0.030).与正常黏膜比较,AMPKα在管状腺瘤组织中表达较低(t=5.545,P=0.014).与正常黏膜及远端黏膜表面上皮分别比较,AMPKα在腺癌组织中表达较低(t=3.159,P=0.006;t=4.503,P=0.047).AMPKα表达与结肠组织病变分类呈正相关(r=0.336,P<0.001),与腺癌不同临床分期无关(r=0.470,P=0.260),与异型增生/上皮内瘤变分级呈负相关(r=-0.453,P=0.028),亦与结肠癌病理分级呈负相关(r=-0.340,P=0.042).结论 伴随正常结肠黏膜上皮的成熟分化,AMPKα的表达呈增加趋势,并在黏膜表面上皮中显著高表达,在结肠腺瘤、腺癌和转移癌中AMPKα表达降低,提示AMPKα可能在抑制结肠腺癌的发生和发展中发挥积极作用.
Abstract
Objective To study the expression and clinicopathological significance of adenosine monophosphate-ac-tivated protein kinase α(AMPKα)in normal colonic mucosa,adenoma,adenocarcinoma and metastatic colon cancer by u-sing colon cancer tissue microarray.Methods The expression of AMPKα protein in colorectal cancer tissue chip was de-tected by SABC immunohistochemistry,and the positive intensity and positive rate were analyzed by Image J software.Results In the normal colonic mucosa,compared with the corresponding crypt gland,the expression of AMPKα in the sur-face epithelium was higher(t=3.053,P=0.030).Compared with the normal mucosa,the expression of AMPKα in the tubular adenoma was lower(t=5.545,P=0.014).Compared with the surface epithelium of normal mucosa and distal mu-cosa,the expression of AMPKα was lower in the adenocarcinoma(t=3.159,P=0.006;t=4.503,P=0.047).The ex-pression of AMPKα was related to the pathological type of colon cancer(r=0.336,P<0.001),different clinical stages of adenocarcinoma cancer(r=0.470,P=0.260),grade of intraepithelial neoplasia(r=-0.453,P=0.028),and grade of colon cancer(r=-0.340,P=0.042).Conclusion With the maturation and differentiation of normal colonic epithelium,the expression of AMPKα tended to increase and significantly up-regulated in the surface mucosal epithelium,while the ex-pression of AMPK α decreased in adenoma,adenocarcinoma and metastatic carcinoma,suggesting that AMPK α may play a positive role in inhibiting the occurrence and development of colonic adenocarcinoma.
关键词
腺苷酸活化蛋白激酶α/结肠腺癌/结肠腺瘤/细胞分化Key words
adenosine monophosphate-activated protein kinase α/colon adenocarcinoma/colon adenoma/cell dif-ferentiation引用本文复制引用
基金项目
国家自然科学基金资助项目(82060155)
宁夏自然科学基金资助项目(NZ15084)
中国科学院"西部之光"人才培养引进计划项目()
宁夏回族自治区大学生创新训练计划备案项目(NXCX2015184)
出版年
2024
NETL
NSTL
万方数据