Preliminary analysis of expression and significance of adenosine monophosphate-acti-vated protein kinase α in the colon cancer based on tissue microarray
Objective To study the expression and clinicopathological significance of adenosine monophosphate-ac-tivated protein kinase α(AMPKα)in normal colonic mucosa,adenoma,adenocarcinoma and metastatic colon cancer by u-sing colon cancer tissue microarray.Methods The expression of AMPKα protein in colorectal cancer tissue chip was de-tected by SABC immunohistochemistry,and the positive intensity and positive rate were analyzed by Image J software.Results In the normal colonic mucosa,compared with the corresponding crypt gland,the expression of AMPKα in the sur-face epithelium was higher(t=3.053,P=0.030).Compared with the normal mucosa,the expression of AMPKα in the tubular adenoma was lower(t=5.545,P=0.014).Compared with the surface epithelium of normal mucosa and distal mu-cosa,the expression of AMPKα was lower in the adenocarcinoma(t=3.159,P=0.006;t=4.503,P=0.047).The ex-pression of AMPKα was related to the pathological type of colon cancer(r=0.336,P<0.001),different clinical stages of adenocarcinoma cancer(r=0.470,P=0.260),grade of intraepithelial neoplasia(r=-0.453,P=0.028),and grade of colon cancer(r=-0.340,P=0.042).Conclusion With the maturation and differentiation of normal colonic epithelium,the expression of AMPKα tended to increase and significantly up-regulated in the surface mucosal epithelium,while the ex-pression of AMPK α decreased in adenoma,adenocarcinoma and metastatic carcinoma,suggesting that AMPK α may play a positive role in inhibiting the occurrence and development of colonic adenocarcinoma.
adenosine monophosphate-activated protein kinase αcolon adenocarcinomacolon adenomacell dif-ferentiation
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