Establishment of HPLC Fingerprint for Xiangqi Shengru Mixture(香芪生乳合剂)
Objective:To establish the HPLC-DAD fingerprint analysis method of Xiangqi Shengru mixture.Methods:The separation was performed on an ACE EXCEL C18-AR(250 mm×4.6 mm,5 μm)with gradient elution of acetonitrile-0.1％phosphoric acid.The flowing rate was 1.0 mL/min.The temperature of column was 35℃and the detection wavelength was 203 nm.The injection concentration was 10 μL.The similarity degree of the fingerprints of 10 batches of Xiangqi Shengru mixture was evaluated by the Chinese Medicine Chromatog-raphy Fingerprint Similarity Evaluation System Software(Version 2004A),respectively.The chromatographic peaks of the control spectrum and the single medicinal material were matched to determine the attribution of common peak,and the components of common peak were identified by mixing standard solution.Results:The similarity between the fingerprint patterns of 10 batches of Xiangqi Shengru mixture and the control spectra was over than 0.96.There were 36 peaks in total,and all the common peaks could be attributed to a single medicinal material.Conclusion:The established HPLC fingerprint of Xiangqi Shengru mixture can reflect the overall characteristics of the multi-components in the compound preparation,which provides a method basis for the quality control of the preparation.
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