Comprehensive Quality Evaluation of Xixiao Liquid(洗消液)Based on Chemometrics Combined with Entropy TOPSIS Analysis and Multi-Component Quantification
Objective:To comprehensively evaluate the quality of Xixiao liquid from different batches.Methods:The HPLC-QAMS was used to establish the content of astilbin,engeletin,hydroxy-α-sanshool,hydroxy-β-sanshool,hydroxy-γ-sanshool,obacunone,matrine,oxysophocarpine,oxymatrine and kochioside Ic in 15 batches of Xixiao liquid.Chemometrics and entropy technique for order preference by similarity to ideal solution(E-TOPSIS)method were used for comprehensive quality evaluation.Results:There was a good linear relationship among 10 ingredients in the determination range(1.88-94.00,1.09-54.50,1.47-73.50,0.74-37.00,0.26-13.00,1.30-65.00,0.82-41.00,0.53-26.50,4.15-207.50,3.26-163.00 μg/mL),with RSD of precision,repeatability and stability tests all lower than 2.0％,and the recoveries were 96.74％-100.11％(RSD＜2.0％,n=9).The samples could be divided into three categories by cluster analysis(CA)and principal component analysis(PCA).And five components were screened out to cause quality differences by orthogonal partial least squares-discriminant analysis(OPLS-DA),including oxymatrine,astilbin,hydroxy-α-sanshool,kochioside Ic and obacunone.E-TOPSIS analy-sisresults showed that S14 had the best quality and S10 quality was interior.Conclusion:The established method can be used for the comprehensive evaluation of the quality of Xixiao liquid.
万方数据
维普
