Study on Quality Standard of Wuzhuyu Gel Paste(吴茱萸凝胶贴膏)
Objective:To establish quality standards for Wuzhuyu Gel paste.Methods:TLC was adopted to identify Wuzhuyu(Evodia rutaecarpa)in Wuzhuyu Gel Paste.HPLC was used to determine evodiamine and rutecarpine in Wuzhuyu Gel paste.The chromatographic column was Inertsil ODS-3.The mobile phase,acetonitrile-H20(53∶47)was used at a rate of 1 mUmin.The column temperature was 28 ℃ and the detection wavelength was set at 215 nm.The paste content,formedness,adhesiveness in Wuzhuyu Gel paste was examined.Results:Wuzhuyu(Evodia rutaecarpa)was identified by TLC,and the TLC spots were clear and negative without interference.There was a good linear relationship for evodiamine and rutecarpinein the range of 0.156 8-0.940 8 μg(r=0.999 5)and 0.103 2-0.619 2 μg(r=0.999 1).RSD of precision,stability and repeatability test was less than 3.0％.The average recoveries were respectively 100.20％and 100.85％with the RSD of 2.05％and 2.16％(n=6).General inspection of preparations meet the requirements.Conclusion:The established qualitative and quantitative analysis method is applicable quality control of Wuzhuyu Gel paste.
Wuzhuyu Gel pasteTLCHPLCcontent determinationquality standard
万方数据
维普
