Objective:To establish the quality standard for Qingbai Pulvis.Methods:TLC was used for qualitative identification of Qingdai(Indigo Naturalis)and Huangbai(Phellodendri Chinensis Cortex)in Qingbai pulvis.HPLC method was used for the content determination of indigo,indigo red and berberine hydrochloride in Qingbai pulvis.Results:The TLC spots of 2 medicinal materials were clear.The linear range of indigo,indigo red and berberine hydrochloride were 46.79-140.37 pg/mL(r=0.999 9),2.55-7.65 μg/mL(r=0.999 8)and 3.55-10.65 μg/mL(r=0.999 8),respectively.The average recoveries were 97.81％(RSD=1.82％),97.60％(RSD=1.32％)and 96.28％(RSD=1.17％),respectively.Conclusion:This simple and specific method can be used for the quality control of Qingbai Pulvis.
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