Screening of Gastric Cancer Related Natural Killer Cell Genes and Potential Prediction of Traditional Chinese Medicine based on WGCNA
Objective:To screen for natural killer cell genes related to gastric cancer through weighted gene co-expression network analysis(WGCNA),explore the correlation between natural killer cell related genes and gastric cancer prognosis,and predict potential therapeutic traditional Chinese medicine.Methods:WGCNA was used to construct co-expression networks of natural killer cell related genes in the TCGA-STAD dataset and GSE84437 dataset for gastric cancer,respectively,to obtain natural killer cell related genes.Obtain prognostic related genes through single factor Cox regression analysis,perform survival analysis on prognostic related genes,and obtain survival related genes using GSE84433 as the validation set.Using the CIBERSORT deconvolution algorithm to calculate the infiltration of survival related genes in 22 types of immune cells.Map prognosis related genes to the Coremine Medical database to obtain potential traditional Chinese medicine,and perform frequency statistics on the four qi,five flavors,meridian differentiation,and efficacy of selected traditional Chinese medicine.Results:Through WGCNA and differential analysis,98 genes related to natural killer cells were screened,and a total of 22 genes related to prognosis were obtained through single factor Cox analysis.Through survival analysis,5 genes were found to be significantly correlated with survival and most immune cells,and were validated i n the GSE84433 dataset.By mapping prognostic related genes to the Coremine Medical database,186 potential therapeutic traditional Chinese medicines were obtained.Among them,10 traditional Chinese medicines such as Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)appeared more frequently,with the main properties being warm and cold,the main flavors being bitter,spicy,and sweet,and the main meridians being liver meridian,lung meridian,stomach meridian,and spleen meridian.The main effects were tonifying deficiency medicine and clearing heat medicine.Conclusion:Five gastric cancer related natural killer cell genes,including IL32,IRF8,INFG,SNX10,and BATF2,are associated with the prognosis of gastric cancer.Traditional Chinese medicines such as Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)may improve the prognosis of gastric cancer by increasing the activity and quantity of natural killer cells.This study reveals that traditional Chinese medicine may improve the survival prognosis of patients by altering the tumor immune microenvironment,providing ideas for subsequent research on traditional Chinese medicine.
万方数据
维普
