首页|复方石榴皮药膜对金黄地鼠皮脂腺斑形态学及雄激素受体表达的影响

复方石榴皮药膜对金黄地鼠皮脂腺斑形态学及雄激素受体表达的影响

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目的:观察复方石榴皮药膜对金黄地鼠皮脂腺斑大小、组织形态及雄激素受体的影响，探讨其在痤疮治疗中的作用机制。方法:将15只天生具有雄激素依赖性的雄性金黄地鼠随机分为空白组、阳性对照组、药膜组，每组5只。分别于两侧皮脂腺斑处涂抹蒸馏水、0。025%维A酸乳膏、复方石榴皮药膜，连续给药28 d，于第0、14、28天测量金黄地鼠双侧皮脂腺斑面积大小并取部分皮脂腺斑行组织病理检测，免疫组织化学检查雄激素受体(AR)表达水平。结果:药膜组给药第14、28天分别与给药前比较，皮脂腺斑面积减小，差异均有统计学意义(P<0。05)。阳性对照组给药第28天与给药第14天比较，皮脂腺斑面积减小，差异有统计学意义(P<0。05)。给药第28天，药膜组与阳性对照组皮脂腺斑面积大小比较，差异有统计学意义(P<0。05)。给药第28天，与空白组比较，药膜组、阳性对照组皮脂腺斑厚度减小，差异均有统计学意义(P<0。05);药膜组、阳性对照组皮脂腺斑AR表达减少，差异均有统计学意义(P<0。05)。结论:复方石榴皮药膜可减小金黄地鼠痤疮模型皮脂腺斑面积、减轻皮脂腺斑厚度，其作用机理可能与抑制雄激素受体表达有关。
Effects of Compound Pomegranate Peel Medicinal Film on the Morphology of Sebaceous Gland Spots and the Expression of Androgen Receptor in Golden Hamster
Objective:To observe the effect of compound pomegranate Peel medicinal film on the size,morphology and androgen receptor of sebaceous gland spots in golden hamster,and to explore its mechanism in the treatment of acne.Methods:Totally 15 male golden hamsters with androgen dependence were randomly divided into blank group,positive control group and medicinal film group,with 5 mice in each group.Distilled water,0.025%retinoic acid cream,and compound pomegranate Peel medicinal film were applied to the sebaceous gland spots on both sides respectively in three groups,for 28 consecutive days.At 0,14 and 28 days after the beginning of administration,the area of bilateral sebaceous glands was measured,and some sebaceous glands were taken for histopathological and immunohistochemical examination of androgen receptor(AR)expression.Results:Compared with before administration,the area of sebaceous gland spots was significantly reduced in medicinal film group on the 14th day and 28th day(P<0.05).Compared with 14th day of administration,the area of sebaceous gland spots decreased significantly in the positive control group on 28th day of administration(P<0.05).After 28 days of drug administration,there was statistically significant difference in the size of sebaceous gland spots between the medicinal film group and positive control group(P<0.05).After 28 days of drug administration,compared with the blank group,the thickness of sebaceous gland spots decreased in medicinal film group and positive control group,and the difference were statistically significant(P<0.05).Compared with the blank group,the expression of AR de-creased in medicinal film group and positive control group,and the difference was statistically significant(P<0.05).Conclusions:Compound pomegranate Peel medicinal film can reduce the area of sebaceous gland spots and the thickness of sebaceous gland in golden hamster acne model,and its mechanism may be related to the inhibition of androgen receptor expression.

acnecompound pomegranate Peel medicinal filmsebaceous glands spotandrogen receptorgolden hamster

张娟娟、朱飞云、吴淑辉、谢小丽、杨逸璇、朱明芳

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湖南中医药大学附属第二中西医结合医院,湖南 浏阳 410300

湖南中医药大学第二附属医院,湖南 长沙 410005

痤疮 复方石榴皮药膜 皮脂腺斑 雄激素受体 金黄地鼠

国家自然科学基金项目湖南省临床医疗技术创新引导项目湖南省中医药科研计划项目湖南中医药大学校级科研基金联合基金湖南省中医药领军人才项目湖南省高层次卫生人才"225"工程培养项目

823052582020SK5150120210932021XJJJ087湘中医药[2021]10号湘卫函[2018]445号

2024

10.13862/j.cn43-1446/r.2024.02.003

中医药导报
湖南省中医药学会 湖南省中医管理局

中医药导报

CSTPCD
影响因子：0.952
ISSN：1672-951X
年,卷(期)：2024.30(2)
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