Experimental Study on the Anti-Inflammatory,Analgesic and Immunoregulatory Effects of Rutong Ruanjians Tablets(乳痛软坚片)
Objective:To explore the pharmacodynamic effects of anti-inflammatory,analgesic and im-munoregulatory effects of Rutong Ruanjians tablets.Methods:The analgesic effect of Rutong Ruanjian tablets was observed through mouse twisting experiment and mouse hot plate experiment,using acetic acid twisting and hot plate pain-causing model,with the number of times of twisting and the value of pain domains as the detection indexes.The anti-inflammatory effect of Rutong Ruanjian tablets was observed through rat granuloma experiment and foot swelling experiment,using the model of granuloma proliferation induced by implantation of cotton ball and inflammation caused by carrageenan gum,with the weight of granulomas and metatarsophalangeal swelling as the detection indexes.In the mouse serum hemolysin experiment and mouse carbon particle contouring experiment,the intraperitoneal injection of 5%sodium chloride solution chicken erythrocyte suspension,and mouse tail vein injection of 20%India ink(0.1 mL/10g)were used to create a mouse immunosuppression model,with serum hemolysin,contouring index(K)and phagocytosis index(α)as the test indicator,to observe the regulation of immunity of Rutong Ruanjian tablets effect.Results:In the torsion experiment,the high dose group of Rutong Ruanjian tablets showed longer torsion latency than model group,while less number of torsions than model group(P<0.01 or P<0.05);The medium dose group of Rutong Ruanjian tablets showed less number of torsions than model group(P<0.05).In the hot plate experiment,the high and medium dose groups of Rutong Ruanjian tablets showed higher 1 h,2 h and 3 h post-drug pain domain values than model group(P<0.01 or P<0.05).In the rat granuloma experiment,the high,medium and low dose groups of Rutong Ruanjian tablets group showed lower granuloma mass than model group(P<0.01 or P<0.05).In the foot swelling experiment of rats,the high dose group of Rutong Ruanjian tablets showed lower swelling degree of the foot and metatarsal than model group at 1,3 and 4 h after inflammation(P<0.05),and the middle dose group of Rutong Ruanjian tablets showed lower swelling degree of the foot and metatarsal than model group at 3 and 4 h after inflammation(P<0.05).In the serum hemolysin experiment,the high dose group of Rutong Ruanjian tablets showed higher hemolysin than model group(P<0.01).In the experiment of carbon granule contouring,the high,medium and low dose groups of Rutong Ruanjian tablets showed higher contouring index and phagocytic index than model group,and the differ-ences were statistically significant(P<0.01 or P<0.05).Conclusion Rutong Ruanjian tablets shows good pharmaco-logical effects of anti-inflammation,analgesia and immunomodulatory.
万方数据
维普
