Study on the Quality of Different Specification and Grade of Baiji(Bletillae Rhizoma)and UPLC Characteristic Spectrum Based on Principal Component Analysis and Grey Correlation Analysis
Objective:To study differences of specific chromatogram among Baiji(Bletillae rhizoma)of different specification and grade,to provide reference for the quality evaluation of Baiji(Bletillae rhizoma).Methods:According to the weight,Baiji(Bletillae rhizoma)was divided into three grades,and the UPLC characteristic spectrum analysis method of Baiji(Bletillae rhizoma)was established.The similarity evaluation,principal component analysis and grey correlation analysis method were used to analyze the differences among different specification and grade.Results:A total of nine characteristic common peaks were identified and one components were identified for the analysis of 16 different specification and grade of Baiji(Bletillae rhizoma).The similarity among different specification and grade of Baiji(Bletillae rhizoma)was 0.393-0.996,and the difference was obvious.According to the result of principal component analysis and grey correlation analysis,the first-class Baiji(Bletillae rhizoma)showed low score and ranking.Conclusion:The characteristic spectrum of Baiji(Bletillae rhizoma)with different specification and grade show certain differences.This study can provide reference for the classification and quality control of Baiji(Bletillae rhizoma)with different specifications and grades.
Baiji(Bletillae rhizoma)pecification and gradeUPLCcharacteristic spectrumprincipal compo-nent analysisgrey correlation analysisquality evaluation