Effect of Tuhuai Dan Siwu Decoction(土槐丹四物汤)Combined with Compound Qinbai Lotion(复方秦柏洗剂)on Inflammatory Factors in Psoriasis
Objective:To observe the effect of oral Tuhuai Dan Siwu decoction combined with topical compound Qinbai lotion on the mitosis of vaginal epithelial cells,imiquimod-induced psoriasis mouse model lesions and ear inflammation in mice,and to study the therapeutic effect of oral Tuhuai Dan Siwu decoction combined with topical compound Qinbai lotion on psoriasis.Methods:The mouse model of psoriasis was established by intragastric administration of estrogen,external application of imiquimod cream on the back and external application of xylene solution on the ear.Different drugs were given for intervention.The mitotic index of vaginal epithelium,total score of PASI on the back skin,degree of ear swelling and expression levels of inflammatory factors were counted.Results:Compound Qinbai lotion group,Tuhuai Dan Siwu decoction group,Tuhuai Dan Siwu decoction+compound Qinbai lotion group,Calcipotriol group showed lower mitotic index of vaginal epithelium and vaginal epithelium of P-STAT3 and STAT3 optical density values than model group(P＜0.05);Compound Qinbai lotion showed higher vaginal epithelium P-STAT3 and STAT3 optical density value than Calcipotriol group(P＜0.05);There was no significant difference in the mitotic index of vaginal epithelium and the optical density of p-STAT3 and STAT3 in vaginal epithelium between Tuhuai Dan Siwu decoction+compound Qinbai lotion group and Calcipotriol group(P＞0.05).After seven days of administration,the Methotrexate group,Tuhuai Dan Siwu decoction group,compound Qinbai lotion group,Tuhuai Dan Siwu decoction+compound Qinbai lotion group showed lower total PASI score and serum STAT3,IFN-γ,IL-17 and IL-23 levels than model group(P＜0.05).The Tuhuai Dan Siwu decoction group and compound Qinbai lotion group showed higher total PASI score and serum levels of STAT3,IFN-γ,IL-17 and IL-23 than Methotrexate group,and the difference was statistically significant(P＜0.05).There was no significant difference in the total score of PASI and serum levels of STAT3,IFN-γ,IL-17 and IL-23 between Tuhuai Dan Siwu decoction+compound Qinbai lotion group and Methotrexate group(P＞0.05).Methotrexate group,Tuhuai Dan Siwu decoction group,compound Qinbai lotion group,Tuhuai Dan Siwu decoction+compound Qinbai lotion group showed lower ear swelling degree and optical density values of IL-6,IL-17,IL-23 and IFN-γ in ear tissue than model group(P＜0.05);Tuhuai Dan Siwu decoction group and compound Qinbai lotion group showed higher ear swelling degree and optical density values of IL-6,IL-17,IL-23 and IFN-γ in ear tissue than Methotrexate group(P＜0.05).There was no statistically significant difference in mice ear swelling degree andoptical density values of IL-6,IL-17,IL-23 and IFN-γ in ear tissue between Tuhuai Dan Siwu decoction+compound Qinbai lotion group and Methotrexate group(P＞0.05).Conclusion:Oral Tuhuai Dansiwu Decoction combined with topical compound Qinbai lotion shows therapeutic effect on psoriasis,which may be related to the inhibition of epithelial cell mitosis,reduction of inflammatory response,inhibition of STAT3 phosphorylation,and reduction of inflammatory factors.
psoriasisinflammatory factorTuhuai Dan Siwu decoctioncompound Qinbai lotionCalcipotriolMethotrexatmice
万方数据
维普
