Effects of Fennel Extract on Hepatic Fibrosis in Mice
Objective:To study the effects of fennel extract on hepatic fibrosis induced by carbon tetra-chloride(CCl4)combined with alcohol in mice.Methods:SPF male mice were randomly divided into model group,fennel low-dose group,fennel medium-dose group and fennel high-dose group after successful modeling by using CCl4 combined with alcohol,eight mice in each group.Another eight mice were selected as control group.After five weeks of drug intervention,the levels of alanine aminotransferase(ALT)and aspartate amino-transferase(AST)in peripheral blood were detected by automatic biochemical analyzer.ELISA was used to detect hyaluronic acid(HA)and laminin(LN).The activity of superoxide dismutase(SOD)and the content of malondi-aldehyde(MDA)in liver tissues were detected by biochemical method.Liver histomorphological changes and collagen fiber staining were observed by liver tissue HE staining and Masson staining.Results:Compared with control group,the levels of ALT,AST,HA,LN and MDA were significantly increased in model group(P＜0.01),while SOD activity was significantly decreased(P＜0.01).HE staining showed disordered arrangement of liver cells,fibrous connective tissue hyperplasia,infiltration of a large number of inflammatory cells,and pseu-dolobular formation in model group;Masson staining showed that there were a large number of collagen fibers proliferate and hyperplastic collagen fibers form fibrous intervals in model group.Compared with model group,the activity of ALT and AST enzymes and the levels of HA and LN significantly decreased in fennel treat-ment groups(P＜0.05 or P＜0.01);The levels of MDA decreased in fennel medium-dose and high-dose groups(P＜0.05),while the SOD activity in liver tissues significantly increased in fennel high-dosel group(P＜0.05).HE staining and Masson Staining showed that fennel low-dose,medium-dose and high-dose groups showed different improvement on the degree of fibrosis,and that fennel high-dose group showed significant improve-ment.Conclusion:Fennel extract can protect liver cells and alleviate liver fibrosis,and reduce the degree of liver fibrosis induced by CCl4 combined with alcohol in mice.
万方数据
维普
