Objective:To optimize the quality standard of Yexiazhu(Phyllanthus urinaria L.)decoction pieces.Methods:Thin layer chromatography(TLC)was used for qualitative identification for Yexiazhu(Phyllanthus urinaria L.)decoction pieces.Microscopy was used to observe the characteristics of the powder and cross-sectional tissue.High-performance liquid chromatography was used to determine the concentrations of gallic acid and corilatine.The concentrations of water,total ash,acid-insoluble ash and ethanol-soluble extractives were measured by the methods described in the 2020 edition of Chinese Pharmacopoeia.Results:The TLC qualitative identification showed that the fuchsia spots from the Yexiazhu(Phyllanthus urinaria L.)decoction pieces existed at the corresponding position compared with the controls.In the microscopic identification,all kinds of tissues and structures contained in the powder and cross section could be used as the identification basis.The results of quantitative analyses showed that the linear equation of gallic acid was Y=15 068X-14 509(r=0.999 8),and that the content was 0.011 0％-0.258 2％.Similarly,the linear equation of corilatine was Y=38265X-7 032(r=0.999 9),and the content was 0.126 7％-0.329 1％.The content of water,total ash,insoluble ash and ethanol-soluble extractives were 9.49％-11.29％,6.02％-9.92％,0.64％-1.67％and 15.73％-30.25％,respectively.Conclusion:This quality standard research could offer technical support for controlling the quality of Yexiazhu(Phyllanthus urinaria L.)decoction pieces accurately and comprehensively.
关键词
叶下珠/薄层鉴别/显微鉴别/含量测定/质量标准
Key words
Yexiazhu(Phyllanthus urinaria L.)/thin layer identification/microscopic identification/content determination/quality standard
维普
万方数据