Discussion on the Mechanism of Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)Medicine Pair in Treatment of Liver Cancer Based on Network Pharmacology and Molecular Docking
Objective:To explore the potential targets and mechanisms of action of Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)medicine pair in the treatment of liver cancer based on network pharmacology and molecular docking methods.Methods:Through the public database,the active ingredients and targets of Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)and the targets of liver cancer were screened,the intersection of molecular targets and disease targets of active ingredients of drugs was drawn,the traditional Chinese medicine compound regulatory network diagram of drug pair-disease-active ingredient-target was constructed,and the protein-protein interaction network diagram was constructed.GO enrichment analysis and KEGG signaling pathway enrichment analysis were performed,and the active ingredients and key targets were verified through molecular docking.Based on the TIMER database,the relationship between key targets and immune cell infiltration was analyzed,and the expression of key targets in tumor tissues and the impact on the clinical prognosis of patients were analyzed through GEPIA database.Results:6 effective ingredients and 70 common targets of Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)were ultimately screened.The core target naringenin has strong affinity with MAPK3,CASP3,AKT1,CAT,ESR1,and PTGS2.The mechanism of action involved multiple pathways.The core targets were associated with multiple immune cells.The expression of MAPK3 in tumor tissues was higher than that in normal tissues,while the ESR1 was the opposite.In survival curve analysis,those with high MAPK3 expression levels had a relatively poor prognosis,and those with high ESR1 expression levels had a relatively better prognosis.It suggests that MAPK3 is an oncogene and ESR1 is a tumor suppressor gene,which has potential value for the clinical prognosis of liver cancer.Conclusion:Jianghuang(Curcumae Longae Rhizoma)and Yujin(Curcumae Radix)medicine pair can exert a synergistic anti-tumor effect with multiple components,multiple targets and multiple pathways by inducing tumor cell apoptosis,inhibiting tumor angiogenesis,and regulating signaling pathways to reduce tumor immune escape.This provides new ideas and clues for further pharmacological research.
万方数据
维普
