The Effect of Jianpi Qinghua Prescription(健脾清化方)on Insulin Sensitivity in Obese Mice Induced by High-Fat Diet Based on Mitochondrial Dynamics of Skeletal Muscle
Objective:To observe the effect of Jianpi Qinghua Prescription on insulin sensitivity in high-fat diet-induced obesity model mice(DIO),and to discuss its mechanism of action based on mitochondrial dynamics of skeletal muscle.Methods:Totally 30 mice were randomly divided into normal group(NOR group,n=6)and model control group(n=24),and the mice in the NOR group were fed with normal diet.The mice in the model control group were established a C57BL/6J obesity model by high-fat diet.The 18 successfully modeled mice were randomly divided into model group(MOD group),Jianpi Qinghua Prescription group(JPQH group)and metformin group(MET group),with 6 mice in each group.And the three groups were treated with normal saline,Jianpi Qinghua Prescription(1.3 g/mL)and metformin(30mg/mL),respectively.After 6 weeks of drug intervention,body weight was weighed.Fasting insulin(FINS),insulin sensitivity,and glucose tolerance were measured,and reverse transcription polymerase chain reaction(Rt-PCR)was used to detect mitotic fusion protein 1(Mfn1)mRNA,mitotic fusion protein 2(Mfn2)mRNA,optic atrophy protein 1(Opa1)mRNA,dynamin related protein 1(Drp1)mRNA,and mitochondrial fission protein 1(Fis1)mRNA in mouse skeletal muscle.The expression levels of Mfn1,Mfn2,Opa1,Fis1,Drp1 and myosin heavy chain(MYH)proteins were detected by Western blotting.Results:After a week of intervention,the JPQH group showed lower body weight than MOD group(P＜0.05).After 2,3,4,5 and 6 weeks of intervention,there was no significant difference in body weight between JPQH group and MOD group(P＞0.05).After 2,3,4,5 and 6 weeks of intervention,the MET group showed lower body weight than MOD group(P＜0.05),and after 1 week of intervention,there was no significant difference in body weight between MAT group and MOD group(P＞0.05).The JPQH group showed lower FINS level than MOD group(P＜0.05),while there was no significant difference in FINS level between JPQH group and MET group(P＞0.05).In the IPITT experiment,the JPQH group showed lower blood glucose levels than MOD group(P＜0.05);The JPQH group showed higher 0-min blood glucose level than MET group(P＜0.05),while there was no significant difference in blood glucose levels at 30,60,90 and 120 min between JPQH group and MET group(P＞0.05).The JPQH group showed lower AUC than MOD group(P＜0.05),while there was no significant difference between JPQH group and MET group(P＞0.05).In the OGTT test,there was no significant difference in the 0 min blood glucose level between JPQH group and MOD group(P＞0.05);The JPQH group showed lower 30,60,90,120 min blood glucose level than MOD group(P＜0.05),while there was no significant difference between JPQH group and MET group(P＞0.05);The JPQH group showed lower AUC than MOD group(P＜0.05),while there was no statistical difference between JPQH group and MET group(P＞0.05).The JPQH group showed lower relative expression of Mfn1 mRNA than MOD group(P＜0.05),while higher than MET group(P＜0.05).The JPQH group showed higher relative expression of Mfn2 mRNA than MOD group and MET group(P＜0.05).The JPQH group showed lower relative expression of Opa1 mRNA than MOD group(P＜0.05),while higher than MET group(P＜0.05).The JPQH group showed higher relative expression of Fis1 mRNA than MOD group(P＜0.05),while lower than MET group(P＜0.05).There was no significant difference in the relative expression of Drp1 mRNA between JPQH group and MOD group(P＞0.05).The JPQH group showed higher relative expression levels of Mfn1 and Mfn2 proteins than MOD group and MET group(P＜0.05).The JPQH group showed lower relative expression levels of Opa1 and Fis1 proteins than MOD group and MET group(P＜0.05).There was no significant difference in the relative expression of Drp1 protein between JPQH group and MOD group(P＞0.05);The JPQH group showed lower relative expression of MYH protein than MOD group(P＜0.05),while there was no significant difference between JPQH group and MET group(P＞0.05).Conclusion:Jianpi Qinghua Prescription can reduce the body weight,improve insulin sensitiv ity and reduce insulin resistance in obese mice induced by high-fat diet.Jianpi Qinghua Prescription may improve skeletal muscle insulin resistance by affecting mitochondrial dynamics in skeletal muscle to stabilize blood glucose fluctuations.
万方数据
维普
