Study on the Effect of Cangfu Daotan Tang(苍附导痰汤)on Endocrine Hormones and the Expression of miRNA-16 and PDCD-4 in Obese PCOS Rats
Objective:To investigate the effect of Cangfu Daotan Tang on the expression of endocrine hormones,microRNA(miRNA)-16 and programmed cell death factor 4(PDCD-4)in obese polycystic ovary syndrome(PCOS)rats.Method:Totally 50 SPF level female SD rats were randomly divided into 5 groups,with 10 rats in each group.The rats were gavaged with Gehuazhi(0.43 g/kg)in positive control group,and low-dose Cangfu Daotan Tang(1.42 g/kg)and high-dose Cangfu Daotan Tang(5.68 g/kg)respectively in low-dose group and high-dose group.And rats were gavaged with an equal amount of physiological saline in model group and normal group.Each group was continuously administered for 14 days.The body weight,endocrine hormone levels,miRNA-16 and PDCD-4 mRNA expression,as well as PDCD-4 protein expression were compared.Results:The model group,positive control group,low-dose group,and high-dose group showed higher body weight than normal group(P＜0.05);The positive control group,low-dose group,and high-dose group showed lower body weight than model group(P＜0.05),and it was dose-dependent.The model group,positive control group,low-dose group,and high-dose group showed lower E2 levels than normal group(P＜0.05),while higher levels of T,FSH,and LH than normal group(P＜0.05);The positive control group,low-dose group,and high-dose group showed higher E2 levels than model group(P＜0.05),while lower levels of T,FSH,and LH than model group(P＜0.05),and there was a dose-dependent relationship.The model group,positive control group,low-dose group,and high-dose group showed lower expression of miRNA-16 mRNA than normal group,while higher expression of PDCD-4 mRNA than normal group(P＜0.05);The positive control group,low-dose group,and high-dose group showed higher expression of miRNA-16 mRNA than model group(P＜0.05),while lower expression of PDCD-4 mRNA than model group(P＜0.05),and it was dose-dependent.The model group,positive control group,low-dose group,and high-dose group showed higher relative expression levels of PDCD-4 protein than normal group(P＜0.05);The positive control group,low-dose group,and high-dose group showed lower relative expression levels of PDCD-4 protein than model group(P＜0.05),and it was dose-dependent.Conclusion:Cangfu Daotan Tang can regulate the levels of endocrine hormones in obese PCOS rats,and its mechanism may be related to the up-regulation of miR-16 expression and down-regulation of PDCD-4 expression.
万方数据
维普
