Effect of Jiangzhi Mai'an Fang(降脂脉安方)on Lipid Metabolism and Inflammation in NAFLD Rats
Objective:To explore the effect of Jiangzhi Mai'an Fang(JZMA)on non-alcoholic fatty liver disease(NAFLD)rats induced by high fat diet and its possible mechanism.Methods:Totally 8 rats were randomly selected from 53 rats as the control group,while the remaining rats were used as the modeling group.Rats were given regular fed in control group,while high fat feed for modeling in modeling group.After successful modeling,40 NAFLD rats were randomly selected and divided into model group,Yisanfu(易善复)group(0.12 g/kg),JZMA low dose(4.95 g/kg)group,JZMA medium dose(9.90 g/kg)group and JZMA high dose(19.80 g/kg)group,with 8 rats in each group.After the last administration,the morphological and pathological changes of liver were observed by HE staining,and the levels of serum triglyceride(TG),cholesterol(CHOL),glutamic pyruvic transaminase(ALT),glutamic oxaloacetic transaminase(AST)and the levels of TG and CHOL in liver tissue were detected.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),free fatty acid(FFA)and C-reactive protein(CRP)in liver tissue were measured by enzyme-linked immunosorbent assay(ELISA).The levels of SREBP-1c mRNA,FASN mRNA,SIRT1 mRNA and PTGS2/COX-2 mRNA in liver tissue were detected by real-time fluorescence quantitative PCR(qRT-PCR),and the protein levels of p-AMPK,FASN,SIRT1,PTGS2/COX-2 and p-NF-K Bp65 in liver tissue were detected by Western blotting.Results:The JZMA medium and high dose groups could reduce the lipid deposition in the liver of NAFLD rats,significantly decrease the levels of serum TG,CHOL,AST and ALT,and decrease the levels of TNF-α,IL-6 and CRP in liver tissue.In addition,the JZMA medium and high dose groups could significantly increase the protein expression level of p-AMPK in the liver of NAFLD rats,and reduce the expression levels of SREBP-lc,FASN and p-NF-κB-p65.Conclusion:Jiangzhi Mai'an Fang can regulate the blood lipid level of NAFLD rats and improve the liver function damage caused by hyperlipidemia,and it may reduce hepatocyte inflammation and alleviate the process of NAFLD by activating AMPK pathway to regulate liver lipid metabolism and inhibiting NF-κ B activity.
万方数据
维普
