The Effect of Ruxiankang Capsule(乳腺康胶囊)on Rats with Mammary Gland Hyperplasia and Experimental Study on Analgesia
Objective:To study the effect of Ruxiankang capsule on rats with mammary hyperplasia and the analgesic effect of Ruxiankang capsule.Methods:The mammary gland hyperplasia model rats were prepared by sequential modeling with estrogen and progesterone.The successful preparation of the model was confirmed by pathology,and they were divided into model control group,Xiaoyao Pill(逍遥丸)group(0.93 g/kg)and Ruxiankang capsule high,medium and low dose groups(0.8 g/kg,0.4 g/kg and 0.2 g/kg).The administration group was given corresponding drugs by oral gavage for intervention.The blank group and model control group were given equal volume distilled water by gavage.After 45 days of continuous administration,the diameter and height of the first and second pairs of nipples were measured,and the levels of serum estradiol(E2),prolactin(PRL)and progesterone(P)were measured by ELISA.The pathological changes of mammary gland were observed under optical microscope.The writhing pain model induced by glacial acetic acid was used to observe the times of writhing reaction,the time(latency)of the first writhing reaction and the analgesic rate of experimental mice in blank group,aspirin group and Ruxiankang capsule high,medium and low dose groups.Results:Compared with the blank group,the E2 increased,while P and E2/P ratio decreased in model control group(P＜0.01);The PRL increased in model control group(P＜0.01);The height and diameter of the first and second pairs of nipples increased in model control group(P＜0.01).Compared with the model control group,the E2 decreased,while P increased in Xiaoyao Pill group and Ruxiankang capsule high and medium dose groups(P＜0.01);The E2/P ratio and PRL decreased in Xiaoyao Pill group and Ruxiankang capsule high and medium dose groups(P＜0.01);The height and diameter of the first and second pairs of nipples decreased in Xiaoyao Pill group and Ruxiankang capsule high and medium dose groups(P＜0.01).The hyperplasia of mammary gland tissue of rats was improved in Ruxiankang capsule high,medium and low dose groups.The epithelial cells of duct were arranged orderly.The number of acini was significantly reduced,and the volume of glandular lobule was significantly reduced.Compared with the blank group,the writhing times were significantly reduced,and the latency was significantly prolonged in aspirin group and Ruxiankang capsule groups(P＜0.01).Aspirin group and Ruxiankang capsule groups showed higher analgesic rate than blank group.The analgesic rates were 59.55％,57.57％and 53.35％respectively in Ruxiankang capsule high,medium and low dose groups.Conclusion:Ruxiankang capsule shows good anti mammary gland hyperplasia and analgesic effect.
万方数据
维普
