The Impact of Isorhamnetin on the Biological Characteristics of Oral Squamous Cell Carcinoma Cells by Mediating miR-454-3p/PIK3R1 Axis
Objective:To investigate the impact of isorhamnetin(ISO)on the biological characteristics of oral squamous cell carcinoma(OSCC)cells and the regulatory mechanism on miR-454-3p/PIK3R1 axis during this process.Methods:MTT method was applied to detect the toxicity of ISO on TSCC1 cells,subsequently,TSCC1 cells were grouped into OSCC group,ISO group,ISO+miR-NC group,and ISO+miR-454-3p mimic group.MTT assay was applied to detect the survival rate of cells in each group;The clone formation experiment was applied to detect the proliferation of cells in each group;Flow cytometry was applied to detect cell apoptosis in each group;Transwell test was applied to detect cell migration and invasion abilities in each group;RT-qPCR was applied to detect the levels of miR-454-3p mRNA and PIK3R1 mRNA of cells in each group;Western blotting was applied to detect the expression of PIK3R1 protein of cells in each group;Double luciferase experiment was applied to verify the targeting relationship between miR-454-3p and PIK3R1.A nude mouse transplanted tumor model was established and divided into model group,ISO group,ISO+agomir-NC group,ISO+miR-454-3p agomir group,with 5 mice in each group.After 15 days of intervention,the tumor weight and volume of mice were measured in each group;RT-qPCR was applied to detect the levels of miR-454-3p mRNA and PIK3R1 mRNA in tumor tissues.Results:In vitro experiments showed that compared with the OSCC group,the cell survival rate,numbers of migrating and invading cells,and expression level of miR-454-3p reduced in the ISO group and ISO+miR-NC group(P＜0.05),and that the apoptosis rate,the expression levels of PIK3R1 mRNA and protein increased(P＜0.05);The miR-454-3p mimic supplementation experiment reversed the inhibitory effect of ISO on OSCC and the expression level of PIK3R1(P＜0.05),at the same time,the double luciferase reporter gene experiment verified that there was a targeting relationship between miR-454-3p and PIK3R1.In vivo experiments showed that compared with the model group,the weight and volume of transplanted tumors decreased in ISO group(P＜0.05),and that the expression levels of miR-454-3p,PIK3R1 mRNA,and miR-454-3p agomir were consistent with in vitro experiments.Conclusion:ISO can inhibit the proliferation,migration,and invasion of OSCC cells,and promote cell apoptosis by regulating the miR-454-3p/PIK3R1 axis.
万方数据
维普
