Objective:To establish an HPLC method for simultaneous determination of gallic acid,stilbene glucoside,emodin-8-O-β-D-glucoside and emodin in Bushen Jiangu Oral Liquid(补肾健骨口服液).Methods:HPLC was used,with Shim pack GIST C18(250 mm×4.6 mm,5 μm)as the chromatographic column.The mobile phase was acetonitrile 0.1％phosphoric acid solution,subjected to gradient elution.The column temperature was 30 ℃.The injection volume was 10 μL.The detection wavelength was 285 nm.Results:The linear relationship of the 4 constituents was good in their respective linear range.The linear relationship was good in the concentra-tion range of 0.036 970～0.735 800 μg,0.205 400～4.108 000 μg,0.014 130～0.282 600 μg,0.004 853～0.097 060 μg.The standard curve equations of gallic acid,stilbene glucoside,emodin-8-O-β-D-glucoside and emodin were Y=2 513.700X-18.223(r=0.998 7),Y=2 165.500X+40.317(r=0.999 8),Y=2 480.400X+0.768(r=0.999 8),Y=3 845.800X+0.778(r=0.999 9).Conclusion:The method is stable,reliable,simple,reproducible and accurate.It can provide reference for the content determination of Bushen Jiangu Oral Liquid(补肾健骨口服液).
维普
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