The Emotional Stress Hormone Receptor GR Regulates the EMT Process of Breast Cancer Cells by Regulating YAP1
Objective:To explore the regulatory mechanism of glucocorticoid receptor(GR)and Yes-associated protein 1(YAP1)on breast cancer cell metastasis,and to provide new ideas for the treatment of breast cancer.Methods:The expression of GR and YAP1 in breast cancer tissues and adjacent tissues were analyzed by immunohistochemistry.MCF-7 cells were divided into si NC group,si GR group and si YAP1 group.The pro-liferation,invasion,migration and apoptosis of MCF-7 cells were analyzed by MTT assay,Transwell assay,cell scratch assay and flow cytometry,respectively.The expression levels of GR and YAP1 were analyzed by Western blotting.Results:Compared with adjacent normal tissues,the expression levels of YAP1 and GR in breast cancer tissues were increased.Compared with the si NC group,the proliferation,invasion and migration abilities of MCF-7 cells were significantly decreased in the si GR group and si YAP1 group,while the apoptosis rate was increased(P＜0.05).Compared with the si NC group,the expression levels of YAP1 and GR in MCF-7 cells were decreased in si GR group(P＜0.05),and the expression level of YAP1 in MCF-7 cells was decreased in si YAP1 group(P＜0.05).Conclusion:The expression levels of YAP1 and GR in breast cancer tissues were increased.After the activation of GR,emotional stress hormones regulate the proliferation,migration,invasion and apoptosis of MCF-7 cells by activating the intracellular YAP1 pathway,and then lead to the metastasis of breast cancer cells.Targeted regulation of YAP1 to inhibit epithelial mesenchyme transition(EMT)of breast cancer can provide a new treatment strategy for breast cancer patients.
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