Study on Quality Value Transmitting of Standard Decoction of Hugan Jiedu Decoction(护肝解毒汤)Based on Fingerprint and Multicomponent Content Determination
Objective:To establish a fingerprint and multi index component content determination method for the standard decoction of Hugan Jiedu decoction,in order to provide reference for the quality evaluation research of its standard decoction and preparation.Methods:According to HPLC combined with a similarity evaluation system for traditional Chinese medicine chromatographic fingerprints,Fingerprints,similarity analysis and identification of common peaks were performed on 15 batches of standard decoction of Hugan Jiedu decoction.A simultaneous detection method was established for 5 indicator components in the decoction,and the transfer rate of each component was calculated.Results:The similarity of the fingerprint spectra of 15 batches of standard decoctions was over 0.90,with 22 common peaks.The control samples were compared and 10 components were identified,including chlorogenic acid,puerarin,paeoniflorin,iquiritigenin,astilbin,gartanin,berberine chloride hydrate,gomisin A,obacunone and fraxinellone.The transfer rates of chlorogenic acid,puerarin,astilbin,berberine chloride hydrate,and gomisin A in the 15 batches of standard decoctions were 48.26％-63.80％,20.30％-31.50％,28.24％-39.11％,2.89％-5.60％,and 13.51％-21.85％respectively.Conclusion:The combination of fingerprinting,extraction rate and determination of the content of the indicator components was used to analyze the quantitative transfer process of the benchmark samples of the Hugan Jiedu decoction,which can initially formulate the quality standard of the benchmark samples of Hugan Jiedu decoction and provide reference for its future development of new drugs.
万方数据
维普
