Quality Evaluation of Baihuasheshecao(Hedyotis Diffusa)by QAMS Multi-Component Quantitative Combined PCA,OPLS-DA and GRA
Objective:To establish a method for the quality evaluation of Baihuasheshecao(Hedyotis Diffusa)by HPLC-QAMS combined with chemometrics and grey relational analysis(GRA).Methods:The analysis was performed on an Alltima C18 column(5.0 μm,250.0 mm×4.6 mm),with a mobile phase consisting of 0.2％phosphoric acid-acetonitrile for gradient elution.The relative correction factors of rutoside and geniposidic acid,geniposide,asperulosidic acid,asperuloside,2-hydroxy-3-methylanthraquinone,1,2-dihydroxy-3-methylanthraquinone,quercetin,kaempferol,oleanolic acid,ursolic acid,stigmasterol and β-sitosterol were calculated and the durability was also investigated.ESM and QAMS methods were used to determine the contents of these 13 components in 16 batches,and then the statistical software was used for chemometrics and GRA.Results:The methodological verification of 13 components met the requirements of Chinese Pharmacopoeia 2020 edition.The average relative correction factors of geniposidic acid,geniposide,asperulosidic acid,asperuloside,2-hydroxy-3-methylan-thraquinone,1,2-dihydroxy-3-methylanthraquinone quercetin,kaempferol,oleanolic acid,ursolic acid,stigmasterol,β-sitosterol and rutoside were 0.948 9,0.703 3,0.782 4,1.135 9,0.584 5,0.800 5,0.893 3,1.068 3,0.740 6,0.864 0,0.674 5 and 0.542 4,respectively.There was no significant difference between the data analyzed by ESM and QAMS method(P＞0.05).The chemometrics method showed that 16 batches of Baihuasheshecao(Hedyotis Diffusa)could be clustered into three categories,showing certain regional differences.Rutoside,asperulosidic acid,geniposide and oleanolic acid were the main potential markers affecting the quality of Baihuasheshecao(Hedyotis Diffusa).Results of GRA method showed that the quality of Baihuasheshecao(Hedyotis Diffusa)in Zhejiang and Jiangxi were the best in all provinces.Conclusion:The established method is convenient and accurate,and it combined with chemometrics and GRA method can be used for the comprehensive evaluation of the quality of Baihuasheshecao(Hedyotis Diffusa).
Baihuasheshecao(Hedyotis Diffusa)HPLC-QAMSchemometricsPCAOPLS-DAgrey relational analysisquality control
万方数据
维普
