Effect of Qiangxin Lishui Prescription(强心利水方)on Myocardial Damage by Regulating Irisin in Adriamycin-Induced Breast Cancer Zebrafish
Objective:To study the effect of Qiangxin Lishui prescription on myocardia damage induced by adriamycin in zebrafish with breast cancer according to regulating Irisin.Methods:Human breast cancer MDA-MB-231 cells were transplanted into zebrafish yolk sac by microinjection to construct the zebrafish breast cancer transplantation model.According to the death of zebrafish,the tolerance concent ration of Qiangxin Lishui prescription to tumor-bearing zebrafish was screened.Tumor-bearing Zebrafish were randomly divided into blank control group,adriamycin group,dextropropimide group,Qiangxin Lishui prescription group and and Shenmai injection(参麦注射液)group,with 30 fish in each group.The blank control group had no drug tbtervention,and the other groups were given adriamycin with mass concentration of 30 mg/L.Dextroproimide group,Qiangxin Lishui prescription group and Shenmai injection group were given corresponding measures.On the eighth days post fertilization(dpf),10 zebrafish were selected from each group.The heart rate and SV-BA distance were separately measured.The heart stroke output and cardiac output were calculated.The pathological changes were observed by hematoxylin-eosin(HE)staining in the zebrafish heart tissues.The indicators of Irisin,pro-atrial natriuretic peptide(Pro-ANP),N-terminal pro-brain natriuretic peptide(NT-pro BNP),cardiac troponin T(cTnT)and high sensitive C reactive protein(hs-CRP)were detected by enzyme-linked immunosorbent assay(ELISA).Results:According to the screening test of zebrafish's maximum tolerance to Qiangxin Lishui prescription,30 mg/L mass concentration was selected.Compared with the blank control group,the cardiac function declined in adriamycin group and Cardiac pathology showed that there were severe pericardial edema and significant enlargement in adriamycin group,meanwhile the myocardial fibers are sparsely arranged and disordered in heart.The Irisin levels decreased significantly in adriamycin group(P＜0.01),while the levels of hs-CRP,Pro-ANP,NT-pro BNP and cTnT2 increased significantly in adriamycin group(P＜0.01).Compared with the adriamycin group,improvements were made in the dextropropimide,Qiangxin Lishui prescription and Shenmai injection groups.The Irisin level increased in dextropropimide,Qiangxin Lishui prescription and Shenmai injection groups(P＜0.01),while the levels of hs-CRP,Pro-ANP,NT-pro BNP and cTnT2 decreased significantly in varying degrees(P＜0.05 or P＜0.01).The Qiangxin Lishui prescription group showed better effects in improving cardiac function than Shenmai injection group(P＜0.05 or P＜0.01).Conclusion:Qiangxin Lishui prescription shows a protective effect on adriamycin-induced myocardia damage in breast cancer zebrafish.The mechanism may be correlated to promoting irisin secretion and reducing myocardial inflammation.
breast cancerQiangxin Lishui prescriptionzebrafishadriamycinmyocardial damageIrisin
万方数据
维普
