Study on Total Flavonoids from Abelmoschus manihot L.Medic.Mediated AKT1 Signaling Pathway to Antagonize Against Lipid Deposition in HepG-2 Cells
Objective:To investigate the intervention effect and mechanism total flavones of Abelmoschus manihot L.Medic.(TFA)on lipid deposition model in HepG-2 cells induced by palmitic acid(PA).Methods:The cultivated human liver derived HepG-2 cells in vitro were treated by PA to construct a cellular lipid deposition model,and then TFA was extracted from Abelmoschus manihot L.Medic.And TFA or protein kinase B(AKT)inhibitors was used as pretreatment intervention agents,performing intervention against PA induced cellular lipid deposition model.Oil red O staining,enzyme-linked immunosorbent assay and Western blotting experiments were used to determine the expression levels of cellular lipid metabolism,AKT1 and adiponectin(APN)signaling pathway proteins.Results:Compared to PA model group,intervention with TFA or AKT inhibitor showed a significant decrease in the levels of cellular lipid deposition(％),free fatty acids(FFAs)and triglycerides(TG),while APN levels increased in HepG-2 cells(P＜0.05).Western blotting experiments showed that the AKT1 and sterol regulatory element binding protein 1(SREBP-1)increased in PA model group,while levels of APN and glycogen synthase kinase 3 β(GSK3 β)decreased(P＜0.05).Compared with PA model group,the intervention with TFA or AKT inhibitor showed the down-regulation of AKT1 while the up-regulation of APN,and its downstream signals SREBP-1 showed decreased expressions while GSK3β up-regulated(P＜0.05).Conclusion:TFA may mediate the AKT1 inhibition and the activation of APN signaling pathway,to antagonize against PA-induced lipid deposition in HepG-2 cells.
total flavonoids from Abelmoschus manihot(L)MedicAKT1APNlipid depositionantagonistic effectsHepG-2 cells
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