Effect of Acupoint Catgut Embedding at Neiguan(PC 6)and Taichong(LR 3)on Myocardial Injury and Energy Metabolism in Spontaneous Prehypertension Rats
Objective:To explore the effect of acupoint catgut embedding at Neiguan(PC 6)and Taichong(LR 3)on myocardial injury and energy metabolism in spontaneously prehypertensive rats.Methods:Totally 164-week-old spontaneously hypertensive(SHR)rats were randomly divided into model group and acupoint catgut embedding group,and 8 Kyoto Wistar(WKY)rats of the same age were selected as the control group.The rats in acupoint catgut embedding group were located at the Neiguan(PC 6)and Taichong(LR 3)acupoints and were underwent acupoint catgut embedding intervention.The rats were treated with grasping fixation intervention in model group and control group.The trial period was 2 weeks.The systolic blood pressure(SBP),diastolic blood pressure(DBP),mean arterial pressure(MAP)and heart rate(HR)were measured,one hour before the intervention and 2,7,and 14 days after the intervention.HE staining was used to observe the pathological changes of myocardial tissue in the anterior wall of the left ventricle of all groups.The structural differences of mitochondria in myocardial tissue of the left ventricular anterior wall of rats were observed un-der electron microscope.The myocardial levels of angiotensin Ⅱ(Ang Ⅱ)were determined by ELISA kits,and the myocardial level of ATP was measured with an ATP kits.Results:Compared with model group,the DBP and MBP were significantly reduced in acupoint catgut embedding group,2,7 and 14 days after the intervention(P＜0.01),and the SBP decreased in acupoint catgut embedding group,7 and 14 days after the acupoint embedding(P＜0.01).There was no statistically significant difference in HR among 3 groups(P＞0.05).In terms of histopathological staining,myocardial cells in model group were in disorder,and the cell volume and gap were significantly increased with inflammatory cell infiltration.Compared with model group,the arrangement of myocardial cells was regular and the transverse diameter of myocardial cells was significantly reduced in acupoint catgut embedding group(P＜0.05),with basically normal intercellular space.In high magnification transmission electron microscopy,the model group displayed disordered mitochondrial arrangement,irregular shapes,and reduced mitochondrial density.The acupoint catgut embedding group showed improvements over the model group.Compared with model group,the level of ATP were increased while the level of Ang Ⅱ were decreased in the left ventricular myocardial tissue in acupoint catgut embedding group(P＜0.05).Conclusion:Catgut embedding at Neiguan(PC 6)and Taichong(LR 3)could regulate myocardial energy metabolism in spontaneous prehypertensive rats,with a positive effect on improving myocardial injury and reducing blood pressure.
spontaneous prehypertensiveprehypertensive stageNeiguan(PC 6)Taichong(LR 3)acupoint embeddingmyocardial injurymyocardial energy metabolismrat
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维普
