摘要
目的:探讨2型糖尿病(T2DM)患者中医证型与外周血巨噬细胞M1和M2表型的相关性.方法:选取102例T2DM患者及20名健康者作为健康对照组,检测122名受试者的空腹血糖(FBG)、糖化血红蛋白(HbA1c)、空腹胰岛素、空腹C肽、巨噬细胞表型及血清炎症因子(IL-1β、IL-6、IL-4、IL-10)的含量在不同证型患者中的差异.并经Pearson分析各组患者巨噬细胞表型与T2DM中医证型的相关性.结果:T2DM患者外周血M1型巨噬细胞百分比与IL-1β、IL-6呈正相关,与IL-4、IL-10呈负相关;M2型巨噬细胞百分比与I L-1β、IL-6呈负相关,与IL-4、IL-10呈正相关.与健康受试者比较,T2DM患者的FBG、HbA1c、空腹胰岛素、空腹C肽、M1型巨噬细胞百分比、M1/M2比值、IL-1β、IL-6水平均显著升高,M2型巨噬细胞百分比、IL-4、IL-10水平均显著下降(P＜0.05).T2DM各中医证型患者的FBG、HbA1c、空腹胰岛素、空腹C肽、M1型巨噬细胞百分比、M1/M2比值、IL-1β、IL-6含量由高到低排序为痰热互结组、血瘀脉络组、阴阳两虚组、肝肾阴虚组、气阴两虚组,而M2型巨噬细胞百分比、IL-4和IL-10水平由低到高排序为痰热互结组、血瘀脉络组、阴阳两虚组、肝肾阴虚组、气阴两虚组.结论:各中医证型的T2DM中,M1型巨噬细胞百分比含量最高的组是痰热互结组,最低的组是气阴两虚组;M2型巨噬细胞百分比含量最高的是气阴两虚组,含量最低的是痰热互结组.T2DM的病情变化可能与巨噬细胞的极化调控有关.
Abstract
Objective:To explore the correlation between traditional Chinese medicine(TCM)syndrome types and peripheral blood macrophage M1 and M2 phenotypes in patients with type 2 diabetes(T2DM).Methods:A total of 102 patients with T2DM who met the inclusion criteria and 20 healthy subjects were selected.Fasting blood glucose(FBG),glycated hemoglobin(HbA1c),serum fasting insulin,serum fasting C-peptide,macrophage phenotypes,and serum inflammatory factors(IL-1[3,IL-6,IL-4,IL-10)were detected in all 122 subjects to investigate the differences in these parameters among different TCM syndrome types.Pearson analysis was used to explore the correlation between macrophage phenotypes and TCM syndrome types in the different groups of patients.Results:The percentage of M1 macrophages in peripheral blood of T2DM patients was positively correlated with IL-1β and IL-6,and negatively correlated with IL-4 and IL-10.The percentage of M2 macrophages is negatively correlated with IL-1β and IL-6,and positively correlated with IL-4 and IL-10.Compared with healthy subjects,T2DM patients exhibited significant increases in FBG,HbA1c,serum fasting insulin,serum fasting C-peptide levels,the percentage of M1 macrophages,M1/M2 ratio,and levels of IL-1β and IL-6,as well as significant decreases in the percentage of M2 macrophages and levels of IL-4 and IL-10(P＜0.05).Among patients with different TCM syndrome types,the order of parameters from high to low was phlegm-heat binding syndrome,blood stasis in collaterals syndrome,yin-yang deficiency syndrome,liver-kidney yin deficiency syndrome,and qi-yin deficiency syndrome,for FBG,HbA1c,serum fasting insulin,serum fasting C-peptide,the percentage of M1 macrophages,M1/M2 ratio,IL-1β,and IL-6.Conversely,the order of parameters from low to high was phlegm-heat binding syndrome,blood stasis in collaterals syndrome,yin-yang deficiency syndrome,liver-kidney yin deficiency syndrome,and qi-yin deficiency syndrome,for the percentage of M2 macrophages as well as for levels of IL-4 and IL-10.Conclusion:The group with the highest percentage of M1 macrophages in patients with T2DM of different TCM syndrome types was the phlegm-heat binding syndrome group,while the group with the lowest percentage was the qi-yin deficiency syndrome group.The group with the highest percentage of M2 macrophages was the qi-yin deficiency syndrome group,while the group with the lowest percentage was the phlegm-heat binding syndrome group.The changes in T2DM may be related to the polarization regulation of macrophages.
基金项目
黑龙江省卫健委科研项目(2021121020019)
黑龙江省中医药学会2022-2024年度青年人才托举工程项目(黑中学会[2022]85号)
黑龙江省级领军人才梯队后备带头人基金项目(黑人社涵[2022]534号)
维普
万方数据