Correlation between Traditional Chinese Medicine Syndrome Types and Peripheral Blood Macrophage Phenotypes in Type 2 Diabetes
Objective:To explore the correlation between traditional Chinese medicine(TCM)syndrome types and peripheral blood macrophage M1 and M2 phenotypes in patients with type 2 diabetes(T2DM).Methods:A total of 102 patients with T2DM who met the inclusion criteria and 20 healthy subjects were selected.Fasting blood glucose(FBG),glycated hemoglobin(HbA1c),serum fasting insulin,serum fasting C-peptide,macrophage phenotypes,and serum inflammatory factors(IL-1[3,IL-6,IL-4,IL-10)were detected in all 122 subjects to investigate the differences in these parameters among different TCM syndrome types.Pearson analysis was used to explore the correlation between macrophage phenotypes and TCM syndrome types in the different groups of patients.Results:The percentage of M1 macrophages in peripheral blood of T2DM patients was positively correlated with IL-1β and IL-6,and negatively correlated with IL-4 and IL-10.The percentage of M2 macrophages is negatively correlated with IL-1β and IL-6,and positively correlated with IL-4 and IL-10.Compared with healthy subjects,T2DM patients exhibited significant increases in FBG,HbA1c,serum fasting insulin,serum fasting C-peptide levels,the percentage of M1 macrophages,M1/M2 ratio,and levels of IL-1β and IL-6,as well as significant decreases in the percentage of M2 macrophages and levels of IL-4 and IL-10(P＜0.05).Among patients with different TCM syndrome types,the order of parameters from high to low was phlegm-heat binding syndrome,blood stasis in collaterals syndrome,yin-yang deficiency syndrome,liver-kidney yin deficiency syndrome,and qi-yin deficiency syndrome,for FBG,HbA1c,serum fasting insulin,serum fasting C-peptide,the percentage of M1 macrophages,M1/M2 ratio,IL-1β,and IL-6.Conversely,the order of parameters from low to high was phlegm-heat binding syndrome,blood stasis in collaterals syndrome,yin-yang deficiency syndrome,liver-kidney yin deficiency syndrome,and qi-yin deficiency syndrome,for the percentage of M2 macrophages as well as for levels of IL-4 and IL-10.Conclusion:The group with the highest percentage of M1 macrophages in patients with T2DM of different TCM syndrome types was the phlegm-heat binding syndrome group,while the group with the lowest percentage was the qi-yin deficiency syndrome group.The group with the highest percentage of M2 macrophages was the qi-yin deficiency syndrome group,while the group with the lowest percentage was the phlegm-heat binding syndrome group.The changes in T2DM may be related to the polarization regulation of macrophages.
type 2 diabetesmacrophagesTCM syndrome typecorrelationM1/M2Pearson analysis
万方数据
维普
