Bioinformatics Analysis of Differentially Expressed Genes and Prediction of Traditional Chinese Medicine in Psoriasis Complicated with Major Depressive Disorders
Objective:To screen the key genes and pathways of psoriasis complicated with major depression disorders by bioinformatics method,to analyze the possible comorbidity mechanism,to explore the key genes with diagnostic value,and to predict the traditional Chinese medicine with potential effects.Methods:The gene data sets of patients with psoriasis and major depressive disorders were downloaded from Gene Expression Omnibus(GEO)database.Differentially expressed genes(DEGs)of those two diseases were screened by limma package,merge DEGs from the two diseases to obtain common differentially expressed genes(co-DEGs),and import the intersecting genes into the metascape database for GO and KEGG enrichment analysis.Key genes were selected based on the protein-protein interaction network constructed by STRING database and Cytoscape 3.9.0 software,and the diagnostic value of key genes was verified by OmicStudio online tool.Finally,the potential therapeutic traditional Chinese medicine was predicted by Coremine Medical platform.Results:A total of 1 psoriasis data set(GSE30999),2 major depressive disorders data sets(GSE19738 and GSE76826)and 2 validation data sets(GSE13355 and GSE38206)were included,resulting in 1554 psoriasis DEGs and 475 severe depression disorders DEGs,and 87 co-DEGs were obtained after intersection.GO and KEGG analysis indicated that co-DEGs were mainly concentrated in 226 biological processes,18 cell structures,34 molecular functions and 19 signaling pathways.9 possible key genes were identified based on protein-protein interaction network,7 among which proved to have diagnostic value,and 46 traditional Chinese medicine with potential curative effects were predicted.Conclusion:The mechanism of psoriasis complicated with major depression disorders may be related to the Nod-like receptor signaling pathway and the RIG-Ⅰ-like receptor signaling pathway.STAT1,DDX58,MX1,GBP1,IRF7,IFIT3,IFI44,RSAD2,and ISG15 may be the key genes in diagnosis and treatment.Traditional Chinese medicine such as Shengdihuang(Radix Rehmanniae),Daqingye(Isatidis Folium),Huangqin(Scutellariae Radix),Ezhu(Curcumae Rhizoma),Yujin(Curcumae Radix),Danggui(Angelicae Sinensis Radix),and Renshen(Ginseng Radix)may be potential drugs for treatment.
psoriasismajor depression disordersgenediagnosistraditional Chinese medicinebioinfor-matics
万方数据
维普
