首页|从肝论治取穴针灸通过调控JAK2/STAT3途径对膝骨关节炎大鼠关节软骨的保护作用

从肝论治取穴针灸通过调控JAK2/STAT3途径对膝骨关节炎大鼠关节软骨的保护作用

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目的:探究从肝论治取穴针灸通过调控JAK2/STAT3途径对膝骨关节炎(OA)大鼠关节软骨的保护作用。方法:60只SD大鼠随机分为对照组、OA组、OA+针灸组，每组20只，通过手术构建右膝OA模型。OA+针灸组大鼠接受从肝论治取穴针灸4周。检测软骨退化情况、关节炎症，以及JAK2/STAT3转录和蛋白水平。结果:OA组大鼠OARSI评分高于对照组(P＜0。05);干预后，OA+针灸组大鼠OARSI评分低于OA组(P＜0。05)。OA组大鼠透明软骨(HC)厚度低于对照组，钙化软骨(CC)厚度高于对照组(P＜0。05);干预后，OA+针灸组大鼠HC厚度高于OA组，CC厚度低于OA组(P＜0。05)。OA组大鼠白细胞介素-1β(IL-1β)、IL-6、JAK2 mRNA、STAT3 mRNA、JAK2蛋白、STAT3蛋白水平均高于对照组(P＜0。05);干预后，OA+针灸组大鼠IL-1β、IL-6、JAK2 mRNA、STAT3 mRNA、JAK2蛋白、STAT3蛋白水平均低于OA组(P＜0。05)。结论:从肝论治取穴针灸可通过调控JAK2/STAT3途径发挥对OA大鼠膝关节软骨的保护作用。
Protective Effect of Acupuncture and Moxibustion on Articular Cartilage in Rats with Knee Osteoarthritis through Regulation of JAK2/STAT3 Pathway Based on Liver Theory and Treatment
Objective:To explore the protective effect of acupuncture and moxibustion on the articular cartilage of rats with knee osteoarthritis(OA)by regulating the JAK2/STAT3 pathway based on liver theory and treatment.Methods:Totally 60 SD rats were randomely divided into control group,OA group,OA+acupuncture group,20 rats in each group.The right knee OA model was surgically constructed.The rats in the OA+acupuncture group received acupuncture at acupoints selected from the liver theory for 4 weeks.Cartilage degeneration,joint inflammation,and JAK2/STAT3 transcription and protein levels were detected.Results:OA group showed higher OARSI score than control group(P＜0.05).OA+acupuncture group showed lower OARSI score than OA group(P＜0.05).OA group showed lower thickness of hyaline cartilage(HC)than control group,while higher thickness of calcified cartilage(CC)than control group(P＜0.05).OA+acupuncture group showed higher thickness of HC than OA group,while lower thickness of CC than OA group(P＜0.05).OA group showed higher IL-1β,IL-6,JAK2 mRNA,STAT3 mRNA,JAK2 protein and STAT3 protein than control group(P＜0.05).OA+acupuncture group showed lower IL-1β,IL-6,JAK2 mRNA,STAT3 mRNA,JAK2 protein and STAT3 protein than OA group(P＜0.05).Conclusion:Acupuncture and moxibustion based on acupoints selected from liver theory play a protective role on knee articular cartilage in knee OA rats by regulating JAK2/STAT3 pathway.

knee osteoarthritistreatment based on liveracupuncture and moxibustionJAK2STAT3cartilagerats

樊远志、张峻峰、陈威、李艳、吴耀持

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上海市第六人民医院,上海 200233

膝骨关节炎 从肝论治 针灸 JAK2 STAT3 软骨 大鼠

上海市名老中医学术经验研究工作室建设项目上海市中医药管理局治未病技术处方与规范项目上海市进一步加快中医药传承创新发展三年行动计划-上海市中西医结合旗舰医院项目

SHGZS-202219ZY2021-2023-0104-02-JS-12ZY2021-2023-0205-04

2024

10.13862/j.cn43-1446/r.2024.07.009

中医药导报
湖南省中医药学会 湖南省中医管理局

中医药导报

CSTPCD
影响因子：0.952
ISSN：1672-951X
年,卷(期)：2024.30(7)