The Mechanism of Compound Qixiong Granule(复方芪芎颗粒)on IL-1βInduced Osteoarthritis Chondrocytes Based on MAPK Signaling Pathway
Objective:To investigate the mechanism of compound Qixiong granule on IL-1β induced rabbit chondrocytes in osteoarthritis based on the MAPK signaling pathway.Methods:Totally five clean New Zealand white rabbits were selected.Chondrocytes were extracted from rabbit cartilage and cultured to the second generation.The chondrocytes were induced with IL-1β to establish an in vitro model of osteoarthritis chondrocytes.The chondrocytes were divided into blank control group,IL-1β group,Diacerein group,low-dose compound Qixong granules group(low-dose group),medium-dose compound Qixong granules group(medium-dose group)and high-dose compound Qixong granules group(high-dose group).The blank control group received no intervention.The IL-1β group was treated with IL-1β at a concentration of 10 μg/L.The Diacerein group was treated with Diacerein at a concentration of 10-5 mol/L.The low,medium,and high-dose groups were treated with 10 mg/mL,20 mg/mL,and 30 mg/mL of compound Qixiong granules,respectively.After intervention for 24,48 and 72 hours,RT-qPCR was used to detect the gene expression levels of P38 kinase,c-Jun N-terminal kinase(JNK),extracellular signal-regulated kinase(ERK),P65 kinase and matrix metalloproteinase 13(MMP-13)in each group.After 72 hours of intervention,the protein expression levels of P38,JNK,ERK,P65 and MMP-13 in each group were detected by Western blotting.Results:After 24,48 and 72 hours of intervention,the IL-1 βgroup showed higher relative expression levels of P38 mRNA,JNK mRNA,ERK mRNA,P65 mRNA and MMP-13 mRNA than blank control group(P＜0.05);The Diacerein group,low-dose group,medium-dose group and high-dose group showed lower relative expression levels of P38 mRNA,JNK mRNA,ERK mRNA,P65 mRNA and MMP-13 mRNA than IL-1β group(P＜0.05);The high-dose group showed lower relative expression levels of P38 mRNA,JNK mRNA,ERK mRNA,P65 mRNA and MMP-13 mRNA than low-dose group(P＜0.05).With the prolongation of drug intervention time,the relative expression levels of P38 mRNA,JNK mRNA,ERK mRNA,P65 mRNA and MMP-13 mRNA showed a decreasing trend in the Diacerein group,low-dose group,medium-dose group and high-dose group(P＜0.05).After 72 hours of intervention,the IL-1β group showed higher relative protein expression levels of P38,JNK,ERK,P65 and MMP-13 than blank control group(P＜0.05);The Diacerein group,low-dose group,medium-dose group and high-dose group showed lower relative protein expression levels of P38,JNK,ERK,P65 and MMP-13 than IL-1β group(P＜0.05);The high-dose group showed lower relative protein expression levels of P38,JNK,ERK,P65 and MMP-13 than low-dose group(P＜0.05).Conclusion:The compound Qixiong granule shows a protective effect on IL-1βinduced osteoarthritis chondrocytes,which may be achieved through the inhibition of the MAPK signaling pathway.Moreover,the inhibitory effect on the MAPK signaling pathway becomes more pronounced with increasing drug concentration and prolonged intervention duration.
osteoarthritiscompound Qixiong granuleinterleukin-1βMAPK signaling pathwaychondrocytesNew Zealand white rabbits
万方数据
维普
