Intervention Effect of Ruhe Neixiao Liquid(乳核内消液)on Hysteromyoma Rats and Its Mechanism Based on Wnt/β-catenin Signaling Pathway
Objective:To investigate the pharmacodynamic effect and mechanism of Ruhe Neixiao Liquid on hysteromyoma rats.Methods:The rat model of uterine leiomyoma was established by estrogen and progesterone loading method.The rats with successful modeling were randomly divided into model group,mifepristone group,Guizhi Fuling(桂枝茯苓)group,Ruhe Neixiao Liquid high dose(4.0 mL/kg)group,Ruhe Neixiao Liquid medium dose(2.0 mL/kg)group and Ruhe Neixiao Liquid low dose(1.0 mL/kg)group.Each group was given corresponding drugs,while the blank group and model group were given equal volume of purified water.After 60 days,the uterus specimens were obtained,and the uterine organ coefficient and smooth muscle thickness index were measured.HE staining was used for pathological observation of uterine tissue.The levels of serum estradiol(E2)and progesterone(P)were detected by ELISA.The expression levels of Wnt and β-catenin in uterine tissues were detected by Western blotting.Results:The high and low doses of Ruhe Neixiao Liquid could significantly reduce the uterine organ coefficient of uterine fibroids model rats,and compared with the model group,the difference was statistically significant(P＜0.05).Ruhe Neixiao Liquid can significantly improve the pathological changes of uterine smooth muscle layer thickening(P＜0.01),smooth muscle arrangement disorder,smooth muscle fiber hyperplasia and hypertrophy.The high,medium and low doses of Ruhe Neixiao Liquid could significantly reduce the levels of serum E2 and P in model rats,and the difference was statistically significant compared with the model group(P＜0.01).The high,medium and low doses of Ruhe Neixiao Liquid could significantly reduce the expression of Wnt-5b protein in uterine tissue of model rats,and the difference was statistically significant compared with the model group(P＜0.05).The high and low doses of Ruhe Neixiao Liquid could significantly reduce the expression of[3-Catenin protein in uterine tissue of model rats,and the difference was statistically significant compared with the model group(P＜0.01).Conclusion:Ruhe Neixiao Liquid can significantly reduce the uterine organ coefficient and sex hormone levels in rats with uterine fibroids,and improve the pathological changes of uterine tissue in rats.The mechanism may be related to the regulation of Wnt/β-catenin signaling pathway.
万方数据
维普
