Effect of Astragalus Polysaccharides on Cell Ppoptosis in Rats with Benign Prostatic Hyperplasia by Regulating the PI3K/AKT/mTOR Signaling Pathway
[Absrtact]Objective:To investigate the effect of Astragalus polysaccharides(APS)on cell apoptosis in rats with benign prostatic hyperplasia(BPH)by regulating the PI3K/AKT/mTOR signaling pathway.Methods:A BPH rat model was established by bilateral testicular removal combined with subcutaneous injection of testosterone propionate.The successfully modeled rats were randomly separated into BPH group,APS low dose(APS low)group(100 mg/kg APS),APS medium dose(APS medium)group(200 mg/kg APS),APS high dose(APS high)group(400 mg/kg APS),and APS high dose+PI3K activator insulin growth factor 1(IGF-1)group(400 mg/kg APS+5 mg/mL IGF-1).After the intervention,an electronic balance was used to weigh the wet weight of the prostate,and the prostate index was calculated.TUNEL staining was applied to detect changes in apoptosis of prostate tissue cells.Immunohistochemistry was applied to detect the expression of the apoptotic gene Caspase-3.Western blotting was applied to detect the expression of pathway related proteins and apoptotic genes Bcl-2 and Bax.Results:Compared with the sham surgery group,the BPH group showed severe pathological damage to the prostate tissue.The prostate wet weight and index,p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR,and Bcl-2 protein expression greatly increased in BPH group,while the apoptosis index,Caspase-3 positive expression,and Bax expression greatly reduced in BPH group(P＜0.05).Compared with the BPH group,the pathological damage was improved in the APS low group,APS medium group,and APS high group.The prostate wet weight and index,p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR,and Bcl-2 protein expression reduced in APS low group,APS medium group,and APS high group,while the apoptosis index,Cacpase-3 positive expression,and Bax expression increased greatly in APS low group,APS medium group,and APS high group.There was statistical difference among different doses of APS(P＜0.05).Compared with the APS high group,the pathological damage was slightly aggravated in APS high+IGF-1 group.The prostate wet weight and index,p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR,and Bcl-2 protein expression increased greatly in APS high+IGF-1 group,while the apoptosis index,Caspase-3 positive expression,and Bax expression reduced greatly in APS high+IGF-1 group(P＜0.05).Conclusion:APS can promote cell apoptosis in BPH rats and effectively inhibit the progression of BPH by inhibiting the PI3K/AKT/mTOR signaling pathway.
万方数据
维普
