Objective:To explore the effects of storage temperature and time on the differentiation of terminal buds and the accumulation of nutrients and lignin in roots of Angelica sinensis seedlings.Methods:Using Angelica sinensis seedlings stored at 0 ℃ and 5 ℃ for 1,2,and 4 months as test materials,observed the changes in their apical buds,and measured the content of soluble sugar,soluble protein,total carbon,total nitrogen,and lignin.Results:The color of the seedlings at 0 ℃ changed from brown to light green with the increase of storage time,and the terminal buds grew and differentiated deeper than those at 5 ℃.The soluble sugar and protein content of seedlings at 0℃ showed a trend of first increasing and then decreasing.At 2 months of storage,0 ℃seedlings contained higher soluble sugar,protein,and lignin content compared to 5 ℃ seedlings.At 4 months of storage,the nutrient content of 0 ℃ seedlings rapidly decreased,lignin accumulation increased,and terminal buds elongated and growed.Grey correlation analysis showed that the C/N ratio and soluble sugar content were key factors affecting lignin accumulation in Angelica sinensis seedlings.Conclusion:At 5 C,Angelica sinensis seedlings could delay bolting and reduced the rate of lignin accumulation in the roots,comprehensively improving their yield and quality.
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