Effect of Xingxiao Pill(醒消丸)on Thyroid Cell Proliferation and Apoptosis in Rats with Nodular Goiter
Objective:To investigate the effect of Xingxiao pill on thyroid cell proliferation and apoptosis in rats with nodular goiter.Methods:The rat model of nodular goiter was replicated by gavage administration of propylthiouracil(PTU).After the model is successfully made,rats were randomly divided into model group,levothyroxine sodium tablets group(15.0 μg/kg),high,medium and low dose Xingxiao pill groups(1.2 g/kg,0.6 g/kg,0.3 g/kg),and a control group was additionally set.After 6 weeks of intervention with corresponding drugs,the weight and relative mass of thyroid tissue were measured.The pathological changes of thyroid tissue were analyzed by HE staining.The levels of thyroid-stimulating hormone(TSH),free triiodothyrogenoaminase(FT3)and free thyroxine(FT4)in serum were detected by ELISA.The apoptosis rate of thyroid cells was analyzed by TUNEL staining.The expression level of VEGF,a proliferation-related protein,was detected by Western blotting.Results:The swelling and proliferation of thyroid follicles in levothyroxine sodium tablets group,the high,medium and low dose Xingxiao pill groups was reduced.The arrangement of epithelial cells tended to be regular in levothyroxine sodium tablets group,and the high,medium and low dose Xingxiao pill groups,and the swelling of thyroid epithelial cells and the infiltration of interstitial inflammatory cells was significantly improved in levothyroxine sodium tablets group,and the high,medium and low dose Xingxiao pill groups.Compared with the model group,the relative weight of thyroid gland in levothyroxine sodium tablets group,and the high dose and low dose Xingxiao pill groups was significantly decreased(P＜0.01),and the weight of thyroid gland in the levothyroxine sodium tablets group and high dose Xingxiao pill groups was significantly reduced(P＜0.01).Compared with the model group,the serum TSH levels of the levothyroxine sodium tablets group,and the high dose and medium dose Xingxiao pill groups were significantly decreased(P＜0.05 or P＜0.01),while the serum FT3 levels of the levothyroxine sodium tablets group,and the high dose and medium dose Xingxiao pill groups were significantly increased(P＜0.01 or P＜0.05).The serum FT4 levels of the levothyroxine sodium tablets group,and the high,medium and low dose Xingxiao pill groups was significantly increased(P＜0.01).Compared with the model group,the apoptosis of thyroid histiocytes in the high and medium dose Xingxiao pill groups was significantly increased(P＜0.05).Compared with the model group,the expression of VEGF protein in the thyroid tissue of the levothyroxine sodium tablet group,and the high and medium dose Xingxiao pill groups was significantly reduced(P＜0.01 or P＜0.05).Conclusion:Xingxiao pill can reduce the content of TSH,increase the content of FT3 and FT4 in the serum of nodular goiter rats,alleviate the changes in thyroid tissue structure of nodular goiter rats,inhibit thyroid cell proliferation,and promote thyroid cell apoptosis.
万方数据
维普
