Determination of Content of Quality Markers of Rhododendron Dauricum L.and Study on Its Optimal Harvesting Period
Objective:To establish a method for determining the content of quality marker based on network pharmacology research for Rhododendron dauricum L.,and evaluate different harvesting periods accordingly.Methods:Agilent Extend-C18 chromatographic column was used,with a flow phase of 0.2％phosphoric acid(A)-acetonitrile solution(B)for gradient elution.The flow rate was 1.0 mL/min,with column temperature of 35 ℃,and the detection wavelength was 360 nm(0-35 min)and 296 nm(35-60 min).The injection amount was 10 μL.Results:There was good linear relationship between peak area and the concentrations of Hyperoside,Quercetin,Kaempferol,8-norrhododendron and rhododendron respectively at 12.32-123.20,20.00-200.00,3.28-32.80,9.32-93.20,120.56-1 205.60 μg/mL.The average recovery rates were respectively from 99.70-103.96％(RSD=1.85％),99.13％-102.61％(RSD=1.71％),98.10％-102.86％(RSD=1.76％),98.29％-100.85％(RSD=0.90％)and 98.76％-100.62％(RSD=0.66％).July is the optimal harvesting period.Conclusions:A HPLC method for the determination of hyperoside,Quercetin,Kaempferol,8-demethylrhododendron and rhododendron was established simultaneously,which makes the quality evaluation of Rhododendron dauricum L more convenient and comprehensive.The best harvest time evaluated by this method is reasonable,providing experimental basis for the secondary processing and application of Rhododendron.
Rhododendron dauricum L.quality markerscontent measurementoptimal harvesting period
万方数据
维普
