摘要
目的:建立满山红药材质量标志物含量测定方法,依此对不同采收时期质量进行评价.方法:采用Agilent Extend-C18色谱柱,流动相为0.2％磷酸(4)-乙腈溶液(B)梯度洗脱,流速为1.0 mL/min,柱温为35 ℃,检测波长为360 nm(0～35 min)和296 nm(35～45 min),进样量为10 μL.结果:金丝桃苷、槲皮素、山柰酚、8-去甲杜鹃素、杜鹃素分别在 12.32～123.20、20.00～200.00、3.28～32.80、9.32～93.20、120.56～1 205.60 µg/mL与峰面积线性关系良好,平均加样回收率分别为 99.70％～103.96％(RSD=1.85％)、99.13％～102.61％(RSD=1.71％)、98.10％～102.86％(RSD=1.76％)、98.29％～100.85％(RSD=0.90％)、98.76％～100.62％(RSD=0.66％);确定7 月份为最佳采收期.结论:采用高效液相色谱(HPLC)法同时建立了金丝桃苷、槲皮素、山柰酚、8-去甲杜鹃素、杜鹃素的测定方法,使满山红的质量评价方法更便捷、更全面,以此评价的最佳采收时期方法合理,可为保证满山红药材质量及促进其开发利用提供实验依据.
Abstract
Objective:To establish a method for determining the content of quality marker based on network pharmacology research for Rhododendron dauricum L.,and evaluate different harvesting periods accordingly.Methods:Agilent Extend-C18 chromatographic column was used,with a flow phase of 0.2％phosphoric acid(A)-acetonitrile solution(B)for gradient elution.The flow rate was 1.0 mL/min,with column temperature of 35 ℃,and the detection wavelength was 360 nm(0-35 min)and 296 nm(35-60 min).The injection amount was 10 μL.Results:There was good linear relationship between peak area and the concentrations of Hyperoside,Quercetin,Kaempferol,8-norrhododendron and rhododendron respectively at 12.32-123.20,20.00-200.00,3.28-32.80,9.32-93.20,120.56-1 205.60 μg/mL.The average recovery rates were respectively from 99.70-103.96％(RSD=1.85％),99.13％-102.61％(RSD=1.71％),98.10％-102.86％(RSD=1.76％),98.29％-100.85％(RSD=0.90％)and 98.76％-100.62％(RSD=0.66％).July is the optimal harvesting period.Conclusions:A HPLC method for the determination of hyperoside,Quercetin,Kaempferol,8-demethylrhododendron and rhododendron was established simultaneously,which makes the quality evaluation of Rhododendron dauricum L more convenient and comprehensive.The best harvest time evaluated by this method is reasonable,providing experimental basis for the secondary processing and application of Rhododendron.
基金项目
黑龙江省中医药管理局中医药科研课题(ZHY2023-062)
维普
万方数据