The Effect of Buzhong Yiqi Jiawei Prescription(补中益气加味方)on Apoptosis of Transplanted Tumor Model of Colorectal Cancer
Objective:To explore the effect of Buzhong Yiqi Jiawei prescription on apoptosis and Caspase-3 expression of transplanted tumor cells in nude mice with colorectal cancer.Methods:Totally 30 nude mice were constructed for the SW480 graft tumor model,and they were randomly divided into chemotherapy group(25 mg/kg intrabitoneal injection of fluorouracil),model group(0.9％sodium chloride solution),Chinese medicine low concentration group(26.72 g/kg),Chinese medicine medium concentration group(53.43 g/kg)and Chinese medicine high concentration group(106.86 g/kg),with 6 mice in each group.Among them,model group,Chinese medicine low concentration group,Chinese medicine medium concentration group and Chinese medicine high concentration group were given intragastric administration to nude mice,once a day for 14 days;The chemotherapy group was given the drug once every other day for 14 days.The nude mice were killed on the 15th day.Statistical comparisons were made for tumor volume and weight of each group.Caspase-3 protein expression was detected by immunohistochemistry method in tumor cells,and TUNEL assay was used to detect apoptosis of tumor cells.Results:There was no significant difference in tumor volume and weight among the 5 groups(P＞0.05);The chemotherapy group,Chinese medicine medium concentration group and Chinese medicine high concentration group showed higher expression of Caspase-3 than model group(P＜0.05);There was no significant difference in expression of Caspase-3 among the chemotherapy group,and Chinese medicine low concentration group,Chinese medicine medium concentration group and Chinese medicine high concentration group(P＞0.05).The Chinese medicine low concentration group,Chinese medicine medium concentration group,Chinese medicine high concentration group and chemotherapy group showed higher apoptosis rate than model group(P＜0.05).The Chinese medicine medium concentration group,Chinese medicine high concentration group and chemotherapy group showed higher apoptosis rate than Chinese medicine low concentration group(P＜0.05);The Chinese medicine medium concentration group and chemotherapy group showed lower apoptosis rate than Chinese medicine high concentration group(P＜0.05);There was no significant difference in apoptosis rate between the Chinese medicine medium concentration group and chemotherapy group(P＞0.05).Conclusion:Buzhong Yiqi Jiawei prescription can improve the apoptosis of tumor cells and the expression of Caspase-3 in nude mice transplanted tumor model of colorectal cancer.
万方数据
维普
