Effects of Intervening TGF-β/Smad Pathway Regulating E-Calmodulin Expression on the Repair of Esophageal Mucosa in Rats with Reflux Esophagitis Model by Quyu Humo Method(祛瘀护膜法)
Objective:To study the effect of Quyu Humo method on the esophageal TGF-β/Smad pathway and E-Cadherin expression in rats with reflux esophagitis.Methods:A total of 56 rats with reflux esophagitis(RE)were established using"4.2 mm pyloric clamp and 2/3 gastric fundus ligation technique".After successful modeling,the rats were randomly divided into Western medicine group 1,Western medicine group 2,treatment group,optimization group,Quyu Humo group and Jiawei Quyu Humo group,with 8 rats in each group.An additional blank group of 8 rats was set up.After continuous administration for 14 days,the rats were sacrificed and the esophageal tissue was removed after blood collection on 15th day.HE staining was used to observe the pathological changes of esophageal tissue under light microscopy;The gap between esophageal epithelial cells were observed with transmission electron microscopy;ELISA method was used to detect transforming growth factor-β1(TGF-β1)and tumor necrosis factor-α(TNF-α);The expression levels of Snail mRNA,Slug mRNA,Twist mRNA and E-Cadherin mRNA were detected by RT-PCR.The levels of TGF-β1,Snail,Slug,Twist,NLRP3,Smad2/3 and p-Smad2/3 were detected by Western blotting.Immunohistochemical staining was used to determine TGF-β1,E-Cadherin and NLRP3.Results:The RE rats treated by the Quyu Humo method generally improved and their body weight increased,with improvement in esophageal mucosal injury;The epithelial cell space tends to shrink;The protein expression of TGF-β1,Snail,Slug,Twist and NLRP3 and Smad2/3 phosphorylation proportions in esophageal tissues were significantly lower than those in the model group(P＜0.05),while the expressions of E-Cadherin and E-Cadherin mRNA were significantly higher than those in the model group.TNF-α and TGF-β1 in serum were significantly lower than those in the model group.Conclusion:The Quyu Humo Method may inhibit the TGF-β/Smad signaling pathway,intervene in the expression of E-cadherin mediated by the pathway,promote the repair of esophagitis mucosa,and antagonize the inflammation of the esophageal mucosa in rats with RE.
万方数据
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