Protective Effect of Jinghua Xiaozi Mixture(荆花消紫合剂)on Purpuric Nephritis in Rats and the Effect on Wnt/β-catenin Pathway-Related Signaling Molecules
Objective:To explore the protective effect of Jinghua Xiaozi mixture on purpuric nephritis in rats and the mechanism of action on Wnt/β-catenin signaling pathway.Methods:A total of 60 healthy male SD rats with SPF grade were selected.Totally 10 rats were randomly selected as the blank group,and the remaining 50 rats were established as experimental IgA nephropathy rat models by combined administration of BSA,LPS and CCl4.The successfully modeled IgA nephropathy rats were randomly divided into model group,Jinghua Xiaozii mixture(high,medium and low)dose group[(10.5 mL/(kg·d),7.0 mL/(kg·d),3.5 mL/(kg·d)],and Western medicine control group[piperazine ferulate tablets 13.5 mg/(kg·d)],10 rats in each group,continuous administration for 4 weeks.Urine analyzer was used to detect urine red blood cell count and 24-hour urine protein quantification in rats.Transmission electron microscopy was used to observe the lesions of kidney tissue in rats.Western blotting was used to detect the protein expressions of Wnt4,Wnt9b,GSK-3β and β-catenin in rat kidney tissues.Real-time fluorescence PCR were used to detect the expressions of Wnt4 mRNA,Wnt9b mRNA,GSK-3β mRNA and β-catenin mRNA in rat kidney tissues.Results:Compared with the blank group,the number of 24-hour urine protein and urine red blood cells were increased significantly in the model group and each drug group(P＜0.05),and the relative expression of Wnt4,Wnt9b,β-catenin protein and Wnt4 mRNA,Wnt9b mRNA,GSK-3β mRNA,β-catenin mRNA in kidney tissue were increased significantly(P＜0.05),while the relative expression of GSK-3β protein was decreased(P＜0.05).Compared with the model group,the number of 24-hour urine protein and urine red blood cells were decreased significantly in each drug group(P＜0.05),and the relative expression of Wnt4,Wnt9b,GSK-3β,β-catenin protein and Wnt4 mRNA,Wnt9b mRNA,GSK-3β mRNA,β-catenin mRNA in kidney tissue were significantly decreased(P＜0.05).Conclusion:Jinghua Xiaozi mixture can effectively reduce the urine red blood cell count and 24-hour urine protein quantification and alleviate kidney injury in rats with henoch-schonlein purpura nephritis,which may be related to the activation of Wnt/β-catenin signaling pathway.
万方数据
维普
