Quantitative Control of Multiple Pharmacodynamic Components in Yinlian Qinggan Mixture(茵莲清肝合剂)by HPLC and Comprehensive Quality Evaluation of Chemometrics Combined with Weighted-Technique for Order Preference by similarity to an Ideal Solution
Objective:To establish a method for evaluating the quality of Yinlian Qinggan mixture by multi-index component quantitative determination combined with chemometrics and weighted-technique for order preference by similarity to an ideal solution(TOPSIS).Methods:HPLC method was used with SunFire C18 column,and the mobile phase was acetonitrile and 0.05％phosphoric acid with gradient elution.The volume flow rate was 1.0 mL/min.Curcumin was used as an internal reference,and the contents of scoparone,albaspidin AA,pseudoaspidin,tetraalbaspidin ABBA,feretoside,asperulosidic acid,asperuloside,bisdemethoxycurcumin,demethoxycurcumin,curcumin,saikosaponin a,saikosaponin d,dehydrotumulosic acid,dehydropachymic acid and pachymic acid in 14 batches of Yinlian Qinggan mixture were determined using external standard method(ESM)and(quantitative analysis of multicomponents by single-marker,QAMS)method.The results obtained from the two methods were compared,and the quality difference of 14 batches of samples was analyzed by statistical software,to find the main potential markers affecting the quality of Yinlian Qinggan mixture.Then,a weighted-TOPSIS model was established based on the VIP value of each component in the stoichiometric analysis and the content data were further processed and analyzed,and then the quality was ranked.Results:The 15 components were well linearly in their respective ranges(r＞0.999),and average added sample recovery rate(n=9)was between 96.93％and 100.14％(RSD＜2.0％).The established relative correction factor showed good durability,and there was no significant difference between the content of each component calculated by the relative correction factor and the content obtained by the external standard method(P＞0.05).The chemometrics method showed that the 14 batches of Yinlian Qinggan mixture could be clustered into three categories,and that adjacent batches were grouped into one group.Scoparone,saikosaponin a,curcumin,feretoside,pachymic acid,saikosaponin d,asperuloside and tetraalbaspidin ABBA were the main factor affecting the quality of Yinlian Qinggan mixture.The weighted-TOPSIS results showed that the euclidean closeness(Ci)of the optimal solution of Yinlian Qinggan mixture in 14 batches ranged from 0.113 6 to 0.792 2,and that the quality of S12 was the best(Ci=0.792 2).Conclusion:The quality evaluation method of Yinlian Qinggan mixture established in this study is convenient and accurate,which can provide reference for scientific evaluation of the quality of Yinlian Qinggan mixture.
万方数据
维普
