Objective:To optimize the extraction process of polysaccharide from Jiangzhi Tongmai Formula(JZTM)by Box-Behnken response surface design.Methods:By comparing extraction method,reflux method,ultrasonic method,ultrasonic-assisted extraction method and ultrasonic-assisted reflux method,it was concluded that ultrasonic-assisted reflux method could improve polysaccharide yield.After that,the influencing factors and the range of JZTM polysaccharide extraction process were optimized based on single factor experiment,and the four-factor and three-level experiment scheme was constructed by Box-Behnk response surface design method,and the optimal process conditions were obtained,and then verified.Results:The extraction rate of polysaccharide by ultrasonic-assisted reflux method was the highest,reaching 12.32 mg/g.Further optimization,the optimum process conditions were as follows:liquid-solid ratio 21.7∶1(mL/g),ultrasonic time 31.19 min,reflux time 2.01h.The extraction rate under this condition was 27.14 mg/g.Conclusion:The extraction process is simple and feasible,and the experimental results are reliable.It provides a reference for the development and utilization of Jiangzhitongmai formula and industrial production.
关键词
降脂通脉方/多糖/超声辅助回流法/响应面法/提取工艺
Key words
Jiangzhi Tongmai Formula/polysaccharides/ultrasound assisted reflux method/extraction process
维普
万方数据