秦川牛mtDNA全基因组遗传多样性与母系起源研究
Genetic Diversity of Mitochondrial DNA Genome and Maternal Origins in Qinchuan Cattle
白萌萌 1马卫东 2成海建 3黄永震 1雷初朝1
作者信息
- 1. 西北农林科技大学动物科技学院,陕西杨凌 712100
- 2. 陕西省农牧良种场,陕西扶风 722203
- 3. 山东省农业科学院畜牧兽医研究所,山东济南 250100
- 折叠
摘要
[目的]从32头秦川牛全基因组中提取其mtDNA全基因组序列进行分析,以揭示秦川牛mtDNA基因组的遗传多样性与母系起源.[方法]采用mtDNA全基因组序列比对及生物信息学方法.[结果]在32头秦川牛mtDNA全基因组序列中,共检测到30种不同的单倍型,其平均单倍型多样度(Hd±SD)为0.996±0.009,其平均核苷酸多样度(π±SD)为0.0067±0.0010,表明秦川牛具有丰富的母系遗传多样性.构建的mtDNA全基因组系统发育树与单倍型网络图表明,32头秦川牛的mtDNA全基因组序列包括T2、T3、T4、I1共4个不同的支系,其中T2支系占21.88％,T3支系占40.625％,T4支系占9.375％,I1支系占28.125％.说明秦川牛具有普通牛和瘤牛两个支系,其中普通牛支系占71.88％,瘤牛支系占28.12％.[结论]秦川牛具有丰富的母系遗传多样性,有普通牛和瘤牛两个母系起源,但以普通牛起源为主.
Abstract
[Objective]This study aimed to investigate the maternal origin and mitochondrial genome genetic diversity of Qinchuan cattle through the analysis of whole mitochondrial genome sequences from 32 Qinchuan cattle samples.[Methods]The study was conducted using mtDNA whole genome alignment and bioinformatics methods.[Results]Analysis revealed a total of 30 different haplotypes within the 32 mtDNA genome sequences of Qinchuan cattle.The average haplotype diversity(Hd±SD)was 0.996±0.009,and the average nucleotide diversity(π±SD)was 0.0067±0.0010,indicating significant maternal genetic diversity in Qinchuan cattle.Phylogenetic tree and haplotype network diagram construction identified four lineages(T2,T3,T4,and I1)among the mtDNA genome sequences,with lineage proportions of 21.88％,40.625％,9.375％,and 28.125％,respectively.Qin-chuan cattle exhibited two main maternal origins,with Bos taurus representing 71.88％and Bos indicus at 28.12％.[Conclusion]This study concludes that Qinchuan cattle possess rich maternal genetic diversity,with two maternal origins of zebu and taurine cattle,albeit taurine cattle being the predominant origin.
关键词
秦川牛/mtDNA全基因组/遗传多样性/母系起源Key words
Qinchuan cattle/mtDNA genome/genetic diversity/maternal origin引用本文复制引用
基金项目
农业农村部国家肉牛牦牛产业技术体系建设项目(CARS-37)
出版年
2024
国家科技期刊平台
NSTL
万方数据