摘要
为调查孟津地区引起奶牛流产的主要病原,本实验采集该地区8个规模化奶牛养殖场共52头新鲜流产胎牛,及其母体的血液、阴道分泌物和鼻腔分泌物.对所采集样本进行新孢子虫(N.caninum)、弓形虫(T.gondii)、胎儿三毛滴虫(T.foetus)、布氏杆菌(B.abor-tus)、牛传染性鼻气管炎病毒(IBRV)和牛病毒性腹泻病毒(BVDV)6种常见流产病原的PCR和RT-PCR检测.结果显示,被检样本总感染率为44.2％(23/52),其中新孢子虫感染率最高,为32.7％(17/52);IBRV次之,为13.5％(7/52);胎儿三毛滴虫、布氏杆菌、弓形虫和BVDV感染率分别为1.9％、1.9％、1.9％和0.11.5％(6/52)的被检样本存在混合感染,其中以新孢子虫+IBRV混合感染为主(50％,3/6).结合被检流产奶牛的临床特征及饲养管理等因素,推断新孢子虫是导致该地区奶牛流产的主要病原.
Abstract
To investigate the main pathogens causing abortion in dairy cows in Mengjin area,this study col-lected samples from 52 fresh aborted fetuses and their maternal blood,vaginal secretions,and nasal secretions from eight large-scale dairy farms in the region.The collected samples were tested for six common abortion-causing pathogens,including Neospora caninum(N.caninum),Toxoplasma gondii(T.gondii),Tritrichomonas foetus(T.foetus),Brucella abortus(B.abortus),Bovine Herpesvirus 1(BoHV-1),and Bovine viral diarrhea virus(BVDV)using PCR and RT-PCR techniques.The results showed that the overall infection rate of the tested sam-ples was 44.2％(23/52),with Neospora caninum having the highest infection rate at 32.7％(17/52);followed by BoHV-1 at 13.5％(7/52);while Tritrichomonas foetus,Brucella abortus,Toxoplasma gondii,and BVDV had respective infection rates of 1.9％,1.9％,1.9％,and 0.Mixed infections were found in 11.5％(6/52)of the tested samples,with Neospora caninum+BoHV-1 being the predominant combination(50％,3/6).Con-sidering the clinical characteristics of the aborted dairy cows and factors related to feeding management,it is in-ferred that Neospora caninum is the primary pathogen causing abortions in this region.
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