To investigate the main pathogens causing abortion in dairy cows in Mengjin area,this study col-lected samples from 52 fresh aborted fetuses and their maternal blood,vaginal secretions,and nasal secretions from eight large-scale dairy farms in the region.The collected samples were tested for six common abortion-causing pathogens,including Neospora caninum(N.caninum),Toxoplasma gondii(T.gondii),Tritrichomonas foetus(T.foetus),Brucella abortus(B.abortus),Bovine Herpesvirus 1(BoHV-1),and Bovine viral diarrhea virus(BVDV)using PCR and RT-PCR techniques.The results showed that the overall infection rate of the tested sam-ples was 44.2％(23/52),with Neospora caninum having the highest infection rate at 32.7％(17/52);followed by BoHV-1 at 13.5％(7/52);while Tritrichomonas foetus,Brucella abortus,Toxoplasma gondii,and BVDV had respective infection rates of 1.9％,1.9％,1.9％,and 0.Mixed infections were found in 11.5％(6/52)of the tested samples,with Neospora caninum+BoHV-1 being the predominant combination(50％,3/6).Con-sidering the clinical characteristics of the aborted dairy cows and factors related to feeding management,it is in-ferred that Neospora caninum is the primary pathogen causing abortions in this region.
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