利用基因组重测序技术鉴定黑安格斯牛的纯度
Assessment of Genetic Purity and Population Genomic Features in Black Angus Cattle Using Whole Genome Re-Sequencing
何亮宏 1王楠 2邓占钊 1李双 2刘学玮 2雷初朝2
作者信息
- 1. 彭阳县畜牧技术推广服务中心,宁夏彭阳 756099
- 2. 西北农林科技大学动物科技学院,陕西杨凌 712100
- 折叠
摘要
[目的]基于10头黑安格斯牛和60头对照组牛的全基因组重测序数据,分析黑安格斯牛的纯度、遗传多样性及群体结构.[方法]全基因组重测序技术和生物信息学方法.[结果]通过对10头黑安格斯牛全基因组数据进行分析,共检测到15,064,459个SNP位点,其核苷酸多样性(pi)为0.0015,观测杂合度(Ho)为0.2381,期望杂合度(He)为0.2430,表明黑安格斯牛的遗传多样性较低;主成分分析和群体遗传结构分析发现,黑安格斯牛与欧洲普通牛聚为一类,其中有4头黑安格斯牛存在偏离情况,表明这4头牛主要与中国瘤牛与东亚普通牛韩牛之间存在杂交.[结论]10头黑安格斯牛中,6头为纯种黑安格斯牛,4头为杂种牛.
Abstract
[Objective]This study utilizes whole-genome resequencing technology to evaluate the genetic integ-rity and diversity of Black Angus cattle.[Methods]Employing whole-genome resequencing data from 10 Black An-gus cattle and 60 control group cattle using bioinformatics tools and analyses,this study investigates the genetic pu-rity,diversity,and population structure.[Results]The whole-genome sequence data analysis of 10 Black Angus cattle revealed 15,064,459 SNP sites.The nucleotide diversity(pi)was recorded at 0.0015,with observed het-erozygosity(Ho)at 0.2381 and expected heterozygosity(He)at 0.2430,indicating a low genetic diversity within the breed.Principal component analysis and population genetic structure assessments showed that Black Angus cat-tle generally cluster with European taurine cattle.Notably,deviations in four Black Angus cattle suggest hybridiza-tion events predominantly with Chinese indicine cattle and East Asian breeds,such as Korean cattle.[Conclusion]Among the cattle examined,six were identified as purebred Black Angus,while four exhibited signs of hybridiza-tion.
关键词
黑安格斯牛/遗传多样性/群体遗传结构/纯度/全基因组Key words
Black Angus cattle/genetic diversity/population genetic structure/purity/whole genome引用本文复制引用
基金项目
国家肉牛牦牛产业技术体系建设项目(CARS-37)
出版年
2024
国家科技期刊平台
NSTL
万方数据