Study on Freezing Effect of Two Amino Acids on Bull Semen
[Objective]This study was to investigate the effect of L-cysteine and arginine on bovine semen freezing.[Method]Different concentrations of L-cysteine and arginine were added to the base solution for tes-ting,and then the optimal ratio was optimized by L9(34)orthogonal table to obtain the different diluent formulas,and then the comparative test was carried out to select the best frozen dilution of bovine semen.[Result]The tha-wing vitality of bovine frozen semen was improved by adding with 0.125%,0.25%and 0.375%cysteine,and 0.5%and 0.75%arginine.[Conclusion]The results showed that cysteine and arginine could be used for the cryopreservation of bovine semen,which could improve the cryopreservation effect of bovine semen,and had obvi-ous effects on improving the quality of bovine frozen semen.
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