两种氨基酸对公牛精液冷冻效果研究
Study on Freezing Effect of Two Amino Acids on Bull Semen
李雯 1杨强 1权约海 1李贵有 1李春平 1李鸿 1朱启耀 1黄必志 2赵家才1
作者信息
- 1. 云南农业职业技术学院
- 2. 云南省草地动物科学研究院,云南昆明 650212
- 折叠
摘要
[目的]试验研究L—半胱氨酸、精氨酸对牛精液冷冻效果.[方法]基础液中分别添加不同浓度的L—半胱氨酸、精氨酸进行试验,再用L9(34)正交表优化最佳配比,获得不同的稀释液配方,然后再进行对比试验,优选出使用效果最好的牛精液冷冻稀释液.[结果]牛精液冷冻稀释液添加0.125%、0.25%、0.375%的半胱氨酸;牛精液冷冻稀释液添加0.5%、0.75%精氨酸,牛冷冻精液解冻活力均得到提高.[结论]结果表明,半胱氨酸、精氨酸可用于牛精液冷冻保存,能改善牛精液冷冻保存效果,对提高牛冷冻精液质量效果明显.
Abstract
[Objective]This study was to investigate the effect of L-cysteine and arginine on bovine semen freezing.[Method]Different concentrations of L-cysteine and arginine were added to the base solution for tes-ting,and then the optimal ratio was optimized by L9(34)orthogonal table to obtain the different diluent formulas,and then the comparative test was carried out to select the best frozen dilution of bovine semen.[Result]The tha-wing vitality of bovine frozen semen was improved by adding with 0.125%,0.25%and 0.375%cysteine,and 0.5%and 0.75%arginine.[Conclusion]The results showed that cysteine and arginine could be used for the cryopreservation of bovine semen,which could improve the cryopreservation effect of bovine semen,and had obvi-ous effects on improving the quality of bovine frozen semen.
关键词
公牛/冷冻精液/稀释液/精氨酸/半光氨酸/试验设计Key words
bull/frozen semen/diluent/arginine/cysteine/experimental design引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据