Population Genetic Structure Analysis of Limousin Cattle in Pengyang County,Ningxia Through Whole Genome Sequencing
[Objective]This study aimed to elucidate the genetic background and lineage purity of 11 Limousin cattle from Pengyang County,Ningxia,by investigating their population genetic structure.[Methods]Whole genome sequencing was performed on 11 Limousin cattle from Pengyang County.Additionally,comparative analysis was con-ducted using 50 cattle samples from published genomic repositories.Bioinformatics techniques,including principal component analysis,phylogenetic tree construction,and ancestral component analysis,were utilized to interpret the data.[Results]Among the 11 Limousin cattle analyzed,10 exhibited mixed ancestry,incorporating genetic compo-nents from East Asian indicine,European taurine,and Chinese zebu cattle.European taurine ancestry was predomi-nant,averaging approximately 60%,followed by East Asian indicine at 28%and Chinese zebu at 12%.This indi-cates varying degrees of hybridization with local Ningxia breeds,specifically Qinchuan and Guyuan cattle.One Lim-ousin cattle was identified as purebred.[Conclusion]To sum up,employing whole genome sequencing and advanced bioinformatics analysis,the genetic composition of Limousin cattle from Pengyang County,Ningxia was characterized.The study identified one purebred Limousin and 10 hybrid cattle,reflecting significant admixture with regional cattle breeds.