首页|利用全基因组测序技术鉴定宁夏彭阳县利木赞牛的群体遗传结构

利用全基因组测序技术鉴定宁夏彭阳县利木赞牛的群体遗传结构

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[目的]通过全基因组测序技术分析宁夏彭阳县 11 头利木赞牛的群体遗传结构及遗传多样性,以揭示其遗传背景和血统纯度。[方法]利用全基因组重测序、主成分分析、系统发育树构建及祖先成分分析等生物信息学方法。[结果]在宁夏彭阳县11 头利木赞牛基因组中,10 头利木赞牛含有东亚普通牛、欧洲普通牛与中国瘤牛血统,其中欧洲普通牛占比最大,平均约占60%,东亚普通牛血统平均约占28%,中国瘤牛血统平均约占12%,表明10头利木赞牛与宁夏地方黄牛群体秦川牛与固原牛存在不同程度的杂交;还有1 头为纯种利木赞牛。[结论]利用全基因组测序技术和生物信息学方法对宁夏彭阳县11 头利木赞牛进行鉴定,其中1 头为纯种利木赞牛,其余10 头均为杂种利木赞牛。
Population Genetic Structure Analysis of Limousin Cattle in Pengyang County,Ningxia Through Whole Genome Sequencing
[Objective]This study aimed to elucidate the genetic background and lineage purity of 11 Limousin cattle from Pengyang County,Ningxia,by investigating their population genetic structure.[Methods]Whole genome sequencing was performed on 11 Limousin cattle from Pengyang County.Additionally,comparative analysis was con-ducted using 50 cattle samples from published genomic repositories.Bioinformatics techniques,including principal component analysis,phylogenetic tree construction,and ancestral component analysis,were utilized to interpret the data.[Results]Among the 11 Limousin cattle analyzed,10 exhibited mixed ancestry,incorporating genetic compo-nents from East Asian indicine,European taurine,and Chinese zebu cattle.European taurine ancestry was predomi-nant,averaging approximately 60%,followed by East Asian indicine at 28%and Chinese zebu at 12%.This indi-cates varying degrees of hybridization with local Ningxia breeds,specifically Qinchuan and Guyuan cattle.One Lim-ousin cattle was identified as purebred.[Conclusion]To sum up,employing whole genome sequencing and advanced bioinformatics analysis,the genetic composition of Limousin cattle from Pengyang County,Ningxia was characterized.The study identified one purebred Limousin and 10 hybrid cattle,reflecting significant admixture with regional cattle breeds.

Limousin cattlewhole genome sequencingpopulation genetic structure

何亮宏、李昱姗、邓占钊、王楠、梁小军、雷初朝

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彭阳县畜牧技术推广服务中心,宁夏彭阳 756099

西北农林科技大学动物科技学院,陕西杨凌 712100

宁夏农林科学院动物科学研究所,银川 750002

利木赞牛 全基因组测序 群体遗传结构

国家肉牛牦牛产业技术体系

CARS-37

2024

中国牛业科学
西北农林科技大学 中国畜牧兽医学会养牛学分会 中国良种黄牛育种委员会

中国牛业科学

影响因子:0.32
ISSN:1001-9111
年,卷(期):2024.50(4)