利用基因组重测序技术鉴定彭阳县夏洛莱牛的纯度
Assessment of Genetic Purity in Charolai Cattle in Pengyang County Using Whole Genome Re-Sequencing
何亮宏 1杨启文 2邓占钊 1王楠 2雷初朝 2梁小军3
作者信息
- 1. 彭阳县畜牧技术推广服务中心,宁夏彭阳 756099
- 2. 西北农林科技大学动物科技学院,陕西杨凌 712100
- 3. 宁夏农林科学院动物科学研究所,银川 750002
- 折叠
摘要
[目的]对宁夏彭阳县10头夏洛莱牛进行全基因组重测序,并与下载的48头牛的全基因组重测序数据进行比对分析,以鉴定彭阳县10头夏洛莱牛的纯度.[方法]利用全基因组重测序技术和生物信息学分析方法.[结果]通过对彭阳县10头夏洛莱牛全基因组数据进行分析,共检测到17,583,444个SNP位点,其基因组的核苷酸多样性(0.0016)稍微高于欧洲普通牛(0.0012)和东亚普通牛(0.0011).遗传结构分析表明,当K=3时,10头彭阳县夏洛莱牛主要为欧洲普通牛血统(64.10％～95.54％),其次是东亚普通牛血统(0.00％～32.25％)和中国瘤牛血统(3.09％～13.51％),说明彭阳县10头夏洛莱牛都存在不同程度的东亚普通牛和中国瘤牛血统.[结论]基于全基因组重测序分析,彭阳县10头夏洛莱牛均为杂交牛.
Abstract
[Objective]To determine the genetic purity of 10 Charolai cattle in Pengyang County,Ningxia,whole genome resequencing was performed and compared with the whole genome resequencing data of 48 cattle downloaded.[Methods]The study utilized whole genome resequencing technology and bioinformatics analysis methods.[Results]A total of 17,583,444 SNP loci were identified through whole-genome analysis of 10 Charolai cattle in Pengyang County.The nucleotide diversity of 10 Charolai cattle genome(0.0016)was found to be a little higher to that of European common cattle(0.0012)and East Asian common cattle(0.0011).The genetic struc-ture analysis showed that when K=3,the 10 Chalolai cattle were mainly European taurine cattle ancestry(64.10％～95.54％),followed by East Asian taurine cattle ancestry(0.00％～32.25％)and Chinese Zebu cattle ancestry(3.09％～13.51％).The results indicated that 10 Charolai cattle in Pengyang County had differ-ent degrees of East Asian taurine cattle and Chinese Zebu cattle ancestry.[Conclusion]The whole genome rese-quencing analysis indicates that all 10 Charolai cattle in Pengyang County are hybrid cattle.
关键词
夏洛莱牛/全基因组重测序/遗传结构/纯度Key words
charolais cattle/whole-genome resequencing/genetic structure/purity引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据