首页|利用基因组重测序技术鉴定彭阳县夏洛莱牛的纯度

利用基因组重测序技术鉴定彭阳县夏洛莱牛的纯度

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[目的]对宁夏彭阳县10头夏洛莱牛进行全基因组重测序,并与下载的48头牛的全基因组重测序数据进行比对分析,以鉴定彭阳县10头夏洛莱牛的纯度。[方法]利用全基因组重测序技术和生物信息学分析方法。[结果]通过对彭阳县10头夏洛莱牛全基因组数据进行分析,共检测到17,583,444个SNP位点,其基因组的核苷酸多样性(0。0016)稍微高于欧洲普通牛(0。0012)和东亚普通牛(0。0011)。遗传结构分析表明,当K=3时,10头彭阳县夏洛莱牛主要为欧洲普通牛血统(64。10%~95。54%),其次是东亚普通牛血统(0。00%~32。25%)和中国瘤牛血统(3。09%~13。51%),说明彭阳县10头夏洛莱牛都存在不同程度的东亚普通牛和中国瘤牛血统。[结论]基于全基因组重测序分析,彭阳县10头夏洛莱牛均为杂交牛。
Assessment of Genetic Purity in Charolai Cattle in Pengyang County Using Whole Genome Re-Sequencing
[Objective]To determine the genetic purity of 10 Charolai cattle in Pengyang County,Ningxia,whole genome resequencing was performed and compared with the whole genome resequencing data of 48 cattle downloaded.[Methods]The study utilized whole genome resequencing technology and bioinformatics analysis methods.[Results]A total of 17,583,444 SNP loci were identified through whole-genome analysis of 10 Charolai cattle in Pengyang County.The nucleotide diversity of 10 Charolai cattle genome(0.0016)was found to be a little higher to that of European common cattle(0.0012)and East Asian common cattle(0.0011).The genetic struc-ture analysis showed that when K=3,the 10 Chalolai cattle were mainly European taurine cattle ancestry(64.10%~95.54%),followed by East Asian taurine cattle ancestry(0.00%~32.25%)and Chinese Zebu cattle ancestry(3.09%~13.51%).The results indicated that 10 Charolai cattle in Pengyang County had differ-ent degrees of East Asian taurine cattle and Chinese Zebu cattle ancestry.[Conclusion]The whole genome rese-quencing analysis indicates that all 10 Charolai cattle in Pengyang County are hybrid cattle.

charolais cattlewhole-genome resequencinggenetic structurepurity

何亮宏、杨启文、邓占钊、王楠、雷初朝、梁小军

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彭阳县畜牧技术推广服务中心,宁夏彭阳 756099

西北农林科技大学动物科技学院,陕西杨凌 712100

宁夏农林科学院动物科学研究所,银川 750002

夏洛莱牛 全基因组重测序 遗传结构 纯度

2024

中国牛业科学
西北农林科技大学 中国畜牧兽医学会养牛学分会 中国良种黄牛育种委员会

中国牛业科学

影响因子:0.32
ISSN:1001-9111
年,卷(期):2024.50(5)