Assessment of Genetic Purity in Charolai Cattle in Pengyang County Using Whole Genome Re-Sequencing
[Objective]To determine the genetic purity of 10 Charolai cattle in Pengyang County,Ningxia,whole genome resequencing was performed and compared with the whole genome resequencing data of 48 cattle downloaded.[Methods]The study utilized whole genome resequencing technology and bioinformatics analysis methods.[Results]A total of 17,583,444 SNP loci were identified through whole-genome analysis of 10 Charolai cattle in Pengyang County.The nucleotide diversity of 10 Charolai cattle genome(0.0016)was found to be a little higher to that of European common cattle(0.0012)and East Asian common cattle(0.0011).The genetic struc-ture analysis showed that when K=3,the 10 Chalolai cattle were mainly European taurine cattle ancestry(64.10%~95.54%),followed by East Asian taurine cattle ancestry(0.00%~32.25%)and Chinese Zebu cattle ancestry(3.09%~13.51%).The results indicated that 10 Charolai cattle in Pengyang County had differ-ent degrees of East Asian taurine cattle and Chinese Zebu cattle ancestry.[Conclusion]The whole genome rese-quencing analysis indicates that all 10 Charolai cattle in Pengyang County are hybrid cattle.