宏基因组测序技术在肉牛疫病检测中的应用研究
Research on the Application of Metagenomic NGS Technology in Detecting Major Diseases of Beef Cattle in Changji Prefecture of Xinjiang
刘文进 1李兆龙 2杨海峰 1赵璐1
作者信息
- 1. 昌吉州动物疾病预防控制中心,新疆 昌吉 831100
- 2. 福建省农业科学院畜牧兽医研究所,福建 福州 350001
- 折叠
摘要
为调查新疆昌吉州地区肉牛主要疫病病原,本试验应用mNGS宏基因组测序技术,对昌吉州地区的肉牛养殖场、活畜交易市场和屠宰场中表现不同临床症状的46 份病样进行检测,结果检出病毒、细菌,血原虫病和支原体等78 种病原,结合当前国内外肉牛养殖过程中已经报道和流行的病原,与宏基因组检测的 46 份临床病例样品的检测结果进行临床比对,筛查出细菌13 种、病毒13 种,以及支原体、血原虫等其他病原 4 种,共计 30 个主要病原,为新疆昌吉肉牛养殖疫病综合防控技术的研究提供科学的数据支撑.
Abstract
In order to investigate the main disease pathogens of beef cattle in Changji Prefecture,Xinjiang,this experiment applied mNGS metagenomic sequencing technology to analyze 46 disease samples with different clinical symptoms from beef cattle farms,live animal trading markets and slaughterhouses in Changji Prefecture.78 patho-gens such as viruses,bacteria,hemoparasiosis and mycoplasma were detected.Combined with the currently repor-ted and prevalent in the current domestic and international beef cattle breeding process,clinical comparisons were made with 46 clinical case samples detected by metagenomic testing.A total of 30 major pathogens were screened,including 13 bacteria,13 viruses,and 4 other pathogens such as mycoplasma and hemochromatosis,providing sci-entific data support for the research of comprehensive disease prevention and control technology in beef cattle breed-ing in Changji,Xinjiang.
关键词
肉牛/宏基因NGS/肉牛疫病Key words
beef cattle/mNGS/beef cattle disease引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据