Preventive effect of Yiqi Huoxue recipe on lipopolysaccharide induced acute lung injury in rats
Objective To observe the preventive effect of Yiqi Huoxue recipe on acute lung injury ( ALI) induced by lipopolysaccharide ( LPS) , and explore its mechanism. Methods Forty healthy male SD rats were randomly divided into normal control group, model control group, dexamethasone group, herbs treatment group, herbs prevention group, eight rats in each group. ALI models were established by intratracheal instillation of LPS (5 mg/kg) while normal control group was instilled normal saline. 3 days before and after making the model,the drug(0. 59g/mL herbs or dexamethasone for 5mg/kg) or same amount of normal saline was given by gavage in each group,and observe their respiration, activity and so on. Rats were sacrificed after 2h of the last administration. Then the lung wet weight / dry weight (W/D) ratio and the protein content in bronchoalveolar lavage fluid ( BALF) was measured, and the pathological changes in lung tissues. Results Compared with normal control group, LPS groups exhibited a higher level of the lung W/D weight ratio, the protein content in BALF and the content of TNF-α, IL-1β, IL-6 in lung tissue (P<0. 01); compared with model control group, the indexes in dexamethasone group and herbs group were decreased (P<0. 01); compared with herbs treatment group, the indexes in herbs prevention group were decreased (P<0. 01 or P<0. 05); the lung W/D weight ratio, the content of TNF-α, IL-1βbetween dexamethasone group and herbs prevention group were close (P>0. 05). Conclusion Yiqi Huoxue recipe can reduce the contents of inflammatory cytokines and protein content in BALF of LPS induced ALI rats, reduce the pathological damage of lung tissue and pulmonary edema, have the effect of prevention and treatment of acute lung injury.
NETL
NSTL
万方数据
维普
