Study the mechanism of supplementing qi and activating blood herbs on Lipopolysaccharide-induced ;acute lung injury in rats
Objective To investigate the effects of supplementing qi and activating blood herbs as-tragalus membranaceus and salvia miltiorrhiza on TLR-4/NF-kappa B signaling pathway in rats with acute lung injury induced by lipopolysaccharide. Methods Forty healthy male SD rats were randomly divided into normal control group, model control group, dexamethasone group, herb group, ten in each group. ALI models were established by intratracheal instillation of LPS ( 5 mg/kg ) , while normal control group was instilled normal saline. 3 days before and after making the model, the drug ( 0. 59 g/mL herbs or dexamethasone for 5 mg/kg) or same amount of normal saline was given in each group by gavage. Rats were sacrificed after 2h of the last administration. Then the protein and mRNA expressions of TLR-4, Interleukin-1 receptor-associated kinase-1 and NF-kappa B were measured by reverse transcriptase polymerase chain reaction and immunohistochemistry respectively. Results Compared with normal control group, the protein and mRNA expressions of LPS groups was higher (P<0. 01); compared with model control group, dexamethasone group and herbs group was decreased(P<0. 01); the expression levels of TLR-4, IRAK-1 in herb group were close to dexamethasone group (P>0. 05),but the level of NF-κB was higher in herb group (P<0. 05) . Conclusion The protective and therapeutic effects of astragalus mem-branaceus and salvia miltiorrhiza on LPS-induced ALI in rats maybe due to its ability to modulate TLR-4/NF-κB signaling pathways.
Supplementing qi and activating blood herbsLipopolysaccharideAcute lung injuryTLR-4IRAK-1NF-κB
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