益肾祛浊方对PCOS模型大鼠胰岛素抵抗及性激素的影响
Effects of Yishen Quzhuo decoction on insulin resistance and hormones of rats with PCOS
蔡云 1许昕1
作者信息
- 1. 100010 首都医科大学附属北京中医医院妇科
- 折叠
摘要
目的 建立由来曲唑联合高脂饲料诱导的多囊卵巢综合征(polycystic ovary syndrome,PCOS)-胰岛素抵抗(insulin resistarce,IR)大鼠模型,观察益肾祛浊方对PCOS大鼠胰岛素抵抗及性激素水平的影响. 方法 将72只6周龄SD雌性大鼠随机分为6组:空白组、模型组、益肾祛浊方高剂量组、益肾祛浊方中剂量组、益肾祛浊方低剂量组及西药二甲双胍组,每组12只.除空白组外,其余各组采用来曲唑联合高脂饲料法建立PCOS-IR大鼠模型.造模成功后,给予灌服益肾祛浊方,以西药二甲双胍作为对照,观察用药后各组大鼠空腹胰岛素、性激素水平及卵巢结构变化. 结果 (1)体重:在造模过程中,除空白组外,各组大鼠体重均明显增加.造模后,经过中药或二甲双胍灌药,各组大鼠与模型组比较体重增长幅度减慢,甚至较前略有减轻.(2)性激素:造模后,模型组黄体生成素(luteinizing hormone,LH)、睾酮(testosterone,T)、黄体生成素/卵泡刺激素(luteinizing hormone/follicle stimulating hormone,LH/FSH)水平较空白组升高;卵泡刺激素(follicle stimulating hormone,FSH)方面,各组无明显差异(P>0.05);用药后益肾祛浊方各剂量组的T、LH、LH/FSH水平较模型组降低,差异有统计学意义(P<0.05),二甲双胍组与模型组比较,T、LH、LH/FSH水平无明显差异(P>0.05).(3)代谢指标:与模型组比较,益肾祛浊方高、中剂量组及西药二甲双胍组的空腹胰岛素(fasting insulin,FINS)水平降低,差异有统计学意义(P<0.05);益肾祛浊方低剂量组与模型组比较FINS水平无明显差异(P>0.05).(4)卵巢组织:益肾祛浊方各剂量组对卵巢多囊样改变均有不同程度改善,使小卵泡数目减少,颗粒细胞层增厚;二甲双胍组改善卵巢组织效果不如益肾祛浊方组明显. 结论 益肾祛浊方能够有效改善PCOS-IR模型大鼠内分泌及代谢紊乱状态,且恢复卵巢内卵泡结构,使颗粒细胞增殖,促进卵泡发育,整体效果优于二甲双胍组.
Abstract
Objective The PCOS-IR rat model was induced by letrozole combined with high fat diet, and the effect of Yishen Quzhuo decoction on insulin resistance and hormones were observed in PCOS rats.Methods 72 6-week-old SD female rats were randomly divided into 6 groups:blank group, model group, Yishen Quzhuo decoction high dose group, Yishen Quzhuo decoction middle dose group, Yishen Quzhuo decoction low dose group, western medicine group, 12 rats in each group.After the rat model was successfully established, the Yishen Quzhuo decoction and western medicine melbine (Metformin Hydrochloride) was used to treat.The levels of insulin and hormones were observed, and the change of ovary structure was observed.Results (1)Effect on the weight of rats: model group was significantly elevated, body weight of rats in each treatment group was decreased after treatment.(2)The influence on testosterone(T), luteinizing hormone(LH), luteinizing hormone/follicle stimulating hormone (LH / FSH): the levels of T and LH / FSH in model group was significantly increased, the levels of T ,LH and LH / FSH in all Yishen Quzhuo decoction groups were reduced.(3)On serum insulin (FINS) in rats: compared with the blank group, the level of FINS of model group was significantly increased, the level of FINS in all treatment groups was decreased except the Yishen Quzhuo decoction low dose group.(4)Ovarian tissue: Yishen Quzhuo decoction groups have different degrees of improvement on polycystic ovary in rats, the number of small follicles was reduced, granular cell layer was thickened;the effect of metformin group was not as obvious as the Chinese medicine group.Conclusion Yishen Quzhuo decoction can effectively improve the endocrine and metabolism of PCOS-IR rats, and can restore the structure of ovarian follicles, can promote the proliferation of granulosa cells and follicular development, the overall effect is better than metformin group.
关键词
多囊卵巢综合征/胰岛素抵抗/中药/益肾祛浊方/二甲双胍Key words
Polycystic ovary syndrome/Insulin resistance/Traditional Chinese medicine/Yishen Quzhuo decoction/Metformin Hydrochloride引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据